Visinin Like Protein 1 Recombinant Protein | VSNL1 recombinant protein
Recombinant Visinin Like Protein 1 (VSNL1)
Gene Names
VSNL1; HLP3; VILIP; HPCAL3; HUVISL1; VILIP-1
Applications
SDS-Page, Western Blot, ELISA, Immunoprecipitation
Purity
>95%
Synonyms
Visinin Like Protein 1; Recombinant Visinin Like Protein 1 (VSNL1); VSNL1 recombinant protein
Host
Host: E Coli
Source: Prokaryotic expression
Source: Prokaryotic expression
Purity/Purification
>95%
Form/Format
Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
Concentration
6.5 (varies by lot)
Sequence
The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGS- PS GRLNLEEFQQ LYVKFFPYGD ASKFAQHAFR TFDKNGDGTI DFREFICALS ITSRGSFEQK LNWAFNMYDL DGDGKITRVE MLEIIEAIYK MVGTVIMMKM NEDGLTPEQR VDKIFSKMDK NKDDQITLDE FKEAAKSDPSIVLL ESLRPHSSTT TTTTEIRLLT KPERKLSWLL PPLSNN
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGS- PS GRLNLEEFQQ LYVKFFPYGD ASKFAQHAFR TFDKNGDGTI DFREFICALS ITSRGSFEQK LNWAFNMYDL DGDGKITRVE MLEIIEAIYK MVGTVIMMKM NEDGLTPEQR VDKIFSKMDK NKDDQITLDE FKEAAKSDPSIVLL ESLRPHSSTT TTTTEIRLLT KPERKLSWLL PPLSNN
Sequence Length
191
Applicable Applications for VSNL1 recombinant protein
SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
Predicted Molecular Mass
24.5kDa
Accurate Molecular Mass (KD)
25kDa
Endotoxin
<1.0EU per 1ug (determined by the LAL method)
Expression System
Prokaryotic expression
Tag
two N-terminal tags, his-tag and T7 tag
Organism Species
Mus musculus (Mouse)
Fragment
Phe225~Thr354
Usage
Reconstitute in sterile PBS, pH7.2-pH7.4.
Preparation and Storage
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
SDS-Page
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
22,142 Da
NCBI Official Full Name
visinin-like protein 1
NCBI Official Synonym Full Names
visinin-like 1
NCBI Official Symbol
VSNL1
NCBI Official Synonym Symbols
HLP3; VILIP; HPCAL3; HUVISL1; VILIP-1
NCBI Protein Information
visinin-like protein 1
UniProt Protein Name
Visinin-like protein 1
Protein Family
UniProt Gene Name
VSNL1
UniProt Synonym Gene Names
VISL1; VILIP; VLP-1; HLP3
UniProt Entry Name
VISL1_HUMAN
NCBI Description
This gene is a member of the visinin/recoverin subfamily of neuronal calcium sensor proteins. The encoded protein is strongly expressed in granule cells of the cerebellum where it associates with membranes in a calcium-dependent manner and modulates intracellular signaling pathways of the central nervous system by directly or indirectly regulating the activity of adenylyl cyclase. Alternatively spliced transcript variants have been observed, but their full-length nature has not been determined. [provided by RefSeq, Jul 2008]
Uniprot Description
VSNL1: Regulates (in vitro) the inhibition of rhodopsin phosphorylation in a calcium-dependent manner. Belongs to the recoverin family.
Chromosomal Location of Human Ortholog: 2p24.3
Molecular Function: calcium ion binding
Biological Process: calcium-mediated signaling
Research Articles on VSNL1
Similar Products
Product Notes
The VSNL1 vsnl1 (Catalog #AAA2030066) is a Recombinant Protein produced from Host: E Coli Source: Prokaryotic expression and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Visinin Like Protein 1 can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP). Researchers should empirically determine the suitability of the VSNL1 vsnl1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.MGSSHHHHH H SSGLVPRGSH MASMTGGQQM GRGS- PS GRLNLEEFQQ LYVKFFPYGD ASKFAQHAFR TFDKNGDGTI DFREFICALS ITSRGSFEQK LNWAFNMYDL DGDGKITRVE MLEIIEAIYK MVGTVIMMKM NEDGLTPEQR VDKIFSKMDK NKDDQITLDE FKEAAKSDPS IVLL ESLRPHSSTT TTTTEIRLLT KPERKLSWLL PPLSNN. It is sometimes possible for the material contained within the vial of "Visinin Like Protein 1, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.
