Protein Vpu (vpu) Recombinant Protein | vpu recombinant protein

Recombinant Human immunodeficiency virus type 1 group M subtype B Protein Vpu (vpu)

Cat. Num. AAA1195803

• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: Protein Vpu (vpu); Recombinant Human immunodeficiency virus type 1 group M subtype B Protein Vpu (vpu); Recombinant Protein Vpu (vpu); Protein Vpu; U ORF protein Viral protein U; vpu recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Positions: 1-34aa; full length protein
• Sequence: ERAEDSGNESEGDHEELSALVDMGHDALWDVDDL
• Sequence Length: 34

• Species: Human immunodeficiency virus type 1 group M subtype B (isolate WMJ22) (HIV-1)
• Preparation and Storage: Store at -20 degree C. For extended storage, store at -20 or -80 degree C.

NCBI and Uniprot Product Information

• NCBI GI #: 139439
• UniProt Accession #: P08808
• Molecular Weight: 3,757 Da
• NCBI Official Full Name: Protein Vpu
• UniProt Protein Name: Protein Vpu
• Protein Family: Protein
• UniProt Gene Name: vpu
• UniProt Entry Name: VPU_HV1W2

Uniprot Description

Function: Enhances virion budding, by targeting human CD4 and Tetherin/BST2 to proteasome degradation. Degradation of CD4 prevents any unwanted premature interactions between viral Env and its receptor human CD4 in the endoplasmic reticulum. Degradation of antiretroviral protein Tetherin/BST2 is important for virion budding, as BST2 tethers new viral particles to the host cell membrane. Mechanistically, Vpu bridges either CD4 or BST2 to BTRC, a substrate recognition subunit of the Skp1/Cullin/F-box protein E3 ubiquitin ligase, induces their ubiquitination and subsequent proteasomal degradation. The alteration of the E3 ligase specificity by Vpu seems to interfere with the degradation of host IKBKB, leading to NF-kappa-B down-regulation and subsequent apoptosis. Ion channel activity has also been suggested, however, formation of cation-selective channel has been reconstituted ex-vivo in lipid bilayers. It is thus unsure that this activity plays a role in vivo

By similarity.

Enzyme regulation: Ion channel activity is inhibited by hexamethylene amiloride in vitro

By similarity.

Subunit structure: May form pentamers or hexamers. Forms ternary complexes, by interacting with human CD4 and BTRC, and with human BST2 and BTRC

By similarity.

Subcellular location: Host membrane; Single-pass type I membrane protein

By similarity.

Domain: The N-terminal and transmembrane domains are required for proper virion budding, whereas the cytoplasmic domain is required for CD4 degradation. The cytoplasmic domain is composed of 2 amphipathic alpha helix

By similarity.

Post-translational modification: Phosphorylated by host CK2. This phosphorylation is necessary for interaction with human BRCP and degradation of CD4

By similarity.

Miscellaneous: HIV-1 lineages are divided in three main groups, M (for Major), O (for Outlier), and N (for New, or Non-M, Non-O). The vast majority of strains found worldwide belong to the group M. Group O seems to be endemic to and largely confined to Cameroon and neighboring countries in West Central Africa, where these viruses represent a small minority of HIV-1 strains. The group N is represented by a limited number of isolates from Cameroonian persons. The group M is further subdivided in 9 clades or subtypes (A to D, F to H, J and K).

Sequence similarities: Belongs to the HIV-1 VPU protein family.