Vanin-1 Recombinant Protein | VNN1 recombinant protein

Recombinant Human Vanin-1

Cat. Num. AAA140997

• Gene Names: VNN1; HDLCQ8; Tiff66
• Purity: Purity as determined by densitometric image analysis is greater than 95%.
• Synonyms: Vanin-1; Recombinant Human Vanin-1; VNN1 Human; Vanin 1 Human Recombinant; Vanin 1; Vascular Non-Inflammatory Molecule 1; Pantetheine Hydrolase; EC 3.5.1.92; HDLCQ8; Tiff66; Pantetheinase; Vannin 1; EC 3.5.1; VNN1; VNN1 recombinant protein

• Host: E Coli
• Purity/Purification: Purity as determined by densitometric image analysis is greater than 95%.
• Form/Format: VNN1 was filtered (0.4 um) and lyophilized in 20mM Tris buffer, 50mM NaCl and 0.1% amisoft CS-22, pH 7.5.; Filtered White lyophilized (freeze-dried) powder.
• Sequence: MKHHHHHHASQDTFTAAVYE HAAILPNATL TPVSREEALA LMNRNLDILE GAITSAADQG AHIIVTPEDA IYGWNFNRDS LYPYLEDIPD PEVNWIPCNN RNRFGQTPVQ ERLSCLAKNN SIYVVANIGD KKPCDTSDPQ CPPDGRYQYN TDVVFDSQGK LVARYHKQNL FMGENQFNVP KEPEIVTFNT TFGSFGIFTC FDILFHDPAV TLVKDFHVDT IVFPTAWMNV LPHLSAVEFH SAWAMGMRVN FLASNIHYPS KKMTGSGIYA PNSSRAFHYD MKTEEGKLLL SQLDSHPSHS AVVNWTSYAS SIEALSSGNK EFKGTVFFDE FTFVKLTGVA GNYTVCQKDL CCHLSYKMSE NIPNEVYALG AFDGLHTVEG RYYLQICTLL KCKTTNLNTC GDSAETASTR FEMFSLSGTF GTQYVFPEVL LSENQLAPGE FQVSTDGRLF SLKPTSGPVL TVTLFGRLYE KDWASNASSG.
• Sequence Length: 513

• Solubility: It is recommended to add 200 ul of deionized water to prepare a working stock solution of approximately 0.5mg/ml and let the lyophilized pellet dissolve completely. VNN1 is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
• Preparation and Storage: Store lyophilized protein at -20 degree C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4 degree C for a limited period of time; it does not show any change after two weeks at 4 degree C.

Related Product Information for VNN1 recombinant protein

Introduction: Vanin 1 (VNN1) belongs to the vanin family of proteins, which share extensive sequence similarity with each other, and also with biotinidase. This family includes secreted and membrane-associated proteins, a few of which have been described to participate in hematopoietic cell trafficking. No biotinidase activity has been established for any of the vanin proteins; nevertheless, they possess pantetheinase activity, which may have a role in oxidative-stress response. VNN1 protein, like its mouse homolog, is probably a GPI-anchored cell surface molecule. The mouse VNN1 protein is expressed by the perivascular thymic stromal cells and regulates migration of T-cell progenitors to the thymus. VNN1 is an amidohydrolase which hydrolyzes specifically one of the carboamide linkages in D-pantetheine thus recycling pantothenic acid (vitamin B5) and releasing cysteamine.

Description: VNN1 Human Recombinant produced in E Coli is a single, non-glycosylated, polypeptide chain (Gln22-Gly491) containing 480 amino acids including a 10 aa His tag at N-terminus. The total calculated molecular mass is 53.5kDa.

Product Categories/Family for VNN1 recombinant protein

Recombinant & Natural Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 223633991
• NCBI GeneID: 8876
• NCBI Accession #: NP_004657.2
• NCBI GenBank Nucleotide #: NM_004666.2
• UniProt Accession #: O95497; Q4JFW6; Q4VAS7; Q4VAS8; Q4VAS9; Q9UF16; Q9UJF4; A8K310
• Molecular Weight: 57,012 Da
• NCBI Official Full Name: pantetheinase
• NCBI Official Synonym Full Names: vanin 1
• NCBI Official Symbol: VNN1
• NCBI Official Synonym Symbols: HDLCQ8; Tiff66
• NCBI Protein Information: pantetheinase
• UniProt Protein Name: Pantetheinase
• Protein Family: Pantetheinase
• UniProt Gene Name: VNN1
• UniProt Synonym Gene Names: Vanin-1
• UniProt Entry Name: VNN1_HUMAN

Uniprot Description

VNN1: Amidohydrolase that hydrolyzes specifically one of the carboamide linkages in D-pantetheine thus recycling pantothenic acid (vitamin B5) and releasing cysteamine. Belongs to the CN hydrolase family. BTD/VNN subfamily.

Protein type: Cell adhesion; Cofactor and Vitamin Metabolism - pantothenate and CoA biosynthesis; EC 3.5.1.92; Hydrolase; Membrane protein, GPI anchor; Membrane protein, integral; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 6q23-q24

Cellular Component: integral to membrane

Molecular Function: pantetheine hydrolase activity

Biological Process: acute inflammatory response; biotin metabolic process; cell motility; cell-cell adhesion; central nervous system development; chronic inflammatory response; inflammatory response; innate immune response; pantothenate metabolic process; positive regulation of T cell differentiation in the thymus; response to oxidative stress