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SDS-Page

Vasoactive Intestinal Peptide (VIP) Recombinant Protein | VIP recombinant protein

Recombinant Vasoactive Intestinal Peptide (VIP)

Gene Names
VIP; PHM27
Applications
SDS-Page, Western Blot
Purity
> 97%
Synonyms
Vasoactive Intestinal Peptide (VIP); Recombinant Vasoactive Intestinal Peptide (VIP); VIP recombinant protein
Ordering
For Research Use Only!
Host
E Coli
Purity/Purification
> 97%
Form/Format
PBS, pH7.4, containing 0.01% SKL.
Concentration
Original Concentration: 200ug/mL (varies by lot)
Sequence
YR APSALRLGDR IPFEGANEPD QVSLKEDIDM LQNALAENDT PYYDVSRNAR HADGVFTSDF SKLLGQLSAK KYLESLMGKR VSSNISEDPV PVKRHSDAVF TDNYTRLRKQ MAVKKYLNSI LNGKRSSEGE SPDFP
Applicable Applications for VIP recombinant protein
Positive Control; Immunogen; SDS-PAGE; Western Blot (WB).
Organism Species
Homo sapiens (Human)
Source
Prokaryotic expression
Residues
Tyr29~Pro165
Tags
N-terminal His Tag
Subcellular Location
Secreted
Traits
Freeze-dried powder
Predicted isoelectric point
6.7
Usage
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Preparation and Storage
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8ºC for one month.
Aliquot and store at -80ºC for 12 months.

Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

SDS-Page

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Predicted Molecular Mass: 16.6kDa
Accurate Molecular Mass: 15/16kDa as determined by SDS-PAGE reducing conditions.
NCBI Official Full Name
VIP peptides isoform 1 preproprotein
NCBI Official Synonym Full Names
vasoactive intestinal peptide
NCBI Official Symbol
VIP
NCBI Official Synonym Symbols
PHM27
NCBI Protein Information
VIP peptides; prepro-VIP
UniProt Protein Name
VIP peptides
Protein Family
UniProt Gene Name
VIP
UniProt Synonym Gene Names
VIP
UniProt Entry Name
VIP_HUMAN

NCBI Description

The protein encoded by this gene belongs to the glucagon family. It stimulates myocardial contractility, causes vasodilation, increases glycogenolysis, lowers arterial blood pressure and relaxes the smooth muscle of trachea, stomach and gall bladder. The protein also acts as an antimicrobial peptide with antibacterial and antifungal activity. Alternative splicing occurs at this locus and two transcript variants encoding distinct isoforms have been identified. [provided by RefSeq, Nov 2014]

Uniprot Description

VIP: VIP causes vasodilation, lowers arterial blood pressure, stimulates myocardial contractility, increases glycogenolysis and relaxes the smooth muscle of trachea, stomach and gall bladder. Belongs to the glucagon family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 6q25

Cellular Component: cell soma; extracellular region

Molecular Function: neuropeptide hormone activity; protein binding; hormone activity

Biological Process: negative regulation of smooth muscle cell proliferation; mRNA stabilization; negative regulation of potassium ion transport; regulation of signal transduction; body fluid secretion; positive regulation of vasodilation; G-protein coupled receptor protein signaling pathway; regulation of protein localization; learning and/or memory; positive regulation of cell proliferation; positive regulation of protein catabolic process; positive regulation of endothelial cell proliferation; regulation of sensory perception of pain; negative regulation of apoptosis

Research Articles on VIP

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Product Notes

The VIP vip (Catalog #AAA2018754) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Vasoactive Intestinal Peptide (VIP) can be used in a range of immunoassay formats including, but not limited to, Positive Control; Immunogen; SDS-PAGE; Western Blot (WB). Researchers should empirically determine the suitability of the VIP vip for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: YR APSALRLGDR IPFEGANEPD QVSLKEDIDM LQNALAENDT PYYDVSRNAR HADGVFTSDF SKLLGQLSAK KYLESLMGKR VSSNISEDPV PVKRHSDAVF TDNYTRLRKQ MAVKKYLNSI LNGKRSSEGE SPDFP. It is sometimes possible for the material contained within the vial of "Vasoactive Intestinal Peptide (VIP), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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