Testing Data
()
DNA Plasmid pUC19 Vector
DNA Plasmid pUC19
Purity
Molecular Biology GradePurified
Synonyms
DNA Plasmid pUC19; DNA Plasmid pUC19 vector
Purity/Purification
Molecular Biology Grade
Purified
Purified
Form/Format
Supplied as a liquid in 10mM Tris HCl, pH 7.6, 1mM EDTA
Preparation and Storage
Store at -20 degree C. Once thawed, keep at 4 degree C. During prolonged storage the supercoiled form may be slowly converted into the relaxed circular form.
Testing Data
()
Testing Data
()
Related Product Information for DNA Plasmid pUC19 vector
pUC19 DNA is isolated from E.coli (dam+, dcm+) and purified by ultracentrifugation twice through a cesium chloride gradient in the presence of ethidium bromide. The molecule is a double-stranded circle, 2686 base pairs in length. GenBank/EMBL accession number L09137.
pUC19 is a plasmid vector suitable for cloning and for the dideoxy DNA sequencing method. It has multiple cloning sites in the lacZ' region. Since the multiple cloning site is inserted into the 5' end of the lac Z' gene, insertion of foreign DNA in this region will destroy the alpha complementation activity of the lac Z' gene product, making the screening for white colonies on plates containing X-Gal and IPTG possible.
Recombinant plasmids generate white colonies while non-recombinant plasmids generate blue colonies.
DNA cloned into pUC19 can be end-labeled while still in the plasmid and sequenced without further DNA purification.
pUC19 is identical to pUC18 except that the polylinker is inserted in the opposite orientation. pUC19 DNA has a molecular weight of 1.8x10e6 daltons and contains 2686 base pairs.
CAP protein binding site - 591-554 (compl. strand); mRNA (LacZ) starts at nt position 507 (compl. strand); lac repressor binding site - 507-487 (compl. strand).
pUC19 is a plasmid vector suitable for cloning and for the dideoxy DNA sequencing method. It has multiple cloning sites in the lacZ' region. Since the multiple cloning site is inserted into the 5' end of the lac Z' gene, insertion of foreign DNA in this region will destroy the alpha complementation activity of the lac Z' gene product, making the screening for white colonies on plates containing X-Gal and IPTG possible.
Recombinant plasmids generate white colonies while non-recombinant plasmids generate blue colonies.
DNA cloned into pUC19 can be end-labeled while still in the plasmid and sequenced without further DNA purification.
pUC19 is identical to pUC18 except that the polylinker is inserted in the opposite orientation. pUC19 DNA has a molecular weight of 1.8x10e6 daltons and contains 2686 base pairs.
CAP protein binding site - 591-554 (compl. strand); mRNA (LacZ) starts at nt position 507 (compl. strand); lac repressor binding site - 507-487 (compl. strand).
Product Categories/Family for DNA Plasmid pUC19 vector
Similar Products
Product Notes
The DNA Plasmid pUC19 (Catalog #AAA641018) is a Vector and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "DNA Plasmid pUC19, Vector" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
