Vitamin D Receptor Blocking Peptide | VDR blocking peptide

Vitamin D Receptor Blocking Peptide

Cat. Num. AAA9617310

• Gene Names: VDR; NR1I1
• Applications: Blocking/Control
• Purity: The purity is >90%,tested by HPLC and MS.
• Synonyms: Vitamin D Receptor; Vitamin D Receptor Blocking Peptide; 1 25 dihydroxyvitamin D3 receptor; 1; 25 dihydroxyvitamin D3 receptor; 25-dihydroxyvitamin D3 receptor; Member 1; NR1I1; Nuclear receptor subfamily 1 group I member 1; PPP1R163; Protein phosphatase 1; regulatory subunit 163; VDR; VDR_HUMAN; Vitamin D (1; 25-dihydroxyvitamin D3) receptor; Vitamin D hormone receptor; Vitamin D nuclear receptor variant 1; Vitamin D receptor; Vitamin D3 receptor; VDR blocking peptide

• Purity/Purification: The purity is >90%,tested by HPLC and MS.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.

• Applicable Applications for VDR blocking peptide: Blocking/Control
• Notes: For phospho antibody, we provide phospho peptide (0.5mg) and non-phospho peptide (0.5mg)
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for VDR blocking peptide

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

NCBI and Uniprot Product Information

• NCBI GI #: 4507883
• NCBI GeneID: 7421
• NCBI Accession #: NP_000367.1
• NCBI GenBank Nucleotide #: NM_000376.2
• UniProt Accession #: P11473; Q5PSV3; B2R5Q1; G3V1V9
• Molecular Weight: 48,289 Da
• NCBI Official Full Name: vitamin D3 receptor isoform VDRA
• NCBI Official Synonym Full Names: vitamin D (1,25- dihydroxyvitamin D3) receptor
• NCBI Official Symbol: VDR
• NCBI Official Synonym Symbols: NR1I1
• NCBI Protein Information: vitamin D3 receptor; 1,25-dihydroxyvitamin D3 receptor; vitamin D nuclear receptor variant 1; nuclear receptor subfamily 1 group I member 1
• UniProt Protein Name: Vitamin D3 receptor
• Protein Family: Vitamin D3 receptor
• UniProt Gene Name: VDR
• UniProt Synonym Gene Names: NR1I1; VDR
• UniProt Entry Name: VDR_HUMAN

NCBI Description

This gene encodes the nuclear hormone receptor for vitamin D3. This receptor also functions as a receptor for the secondary bile acid lithocholic acid. The receptor belongs to the family of trans-acting transcriptional regulatory factors and shows sequence similarity to the steroid and thyroid hormone receptors. Downstream targets of this nuclear hormone receptor are principally involved in mineral metabolism though the receptor regulates a variety of other metabolic pathways, such as those involved in the immune response and cancer. Mutations in this gene are associated with type II vitamin D-resistant rickets. A single nucleotide polymorphism in the initiation codon results in an alternate translation start site three codons downstream. Alternative splicing results in multiple transcript variants encoding different proteins. [provided by RefSeq, Feb 2011]

Uniprot Description

VDR: Nuclear hormone receptor. Transcription factor that mediates the action of vitamin D3 by controlling the expression of hormone sensitive genes. Regulates transcription of hormone sensitive genes via its association with the WINAC complex, a chromatin-remodeling complex. Recruited to promoters via its interaction with the WINAC complex subunit BAZ1B/WSTF, which mediates the interaction with acetylated histones, an essential step for VDR-promoter association. Plays a central role in calcium homeostasis. Defects in VDR are the cause of rickets vitamin D- dependent type 2A (VDDR2A). A disorder of vitamin D metabolism resulting in severe rickets, hypocalcemia and secondary hyperparathyroidism. Most patients have total alopecia in addition to rickets. Belongs to the nuclear hormone receptor family. NR1 subfamily.

Protein type: Nuclear receptor; DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 12q13.11

Cellular Component: nucleoplasm; nucleus; receptor complex

Molecular Function: retinoid X receptor binding; protein binding; DNA binding; zinc ion binding; sequence-specific DNA binding; steroid hormone receptor activity; vitamin D3 receptor activity; transcription factor activity

Biological Process: lactation; transcription initiation from RNA polymerase II promoter; cell morphogenesis; positive regulation of apoptosis involved in mammary gland involution; negative regulation of transcription from RNA polymerase II promoter; decidualization; signal transduction; intestinal absorption; cellular calcium ion homeostasis; negative regulation of cell proliferation; calcium ion transport; positive regulation of transcription from RNA polymerase II promoter; steroid hormone mediated signaling; gene expression; negative regulation of transcription, DNA-dependent; skeletal development; positive regulation of keratinocyte differentiation

Disease: Vitamin D-dependent Rickets, Type 2a; Osteoporosis