Rat Versican ELISA Kit | VCAN elisa kit

Rat Versican ELISA Kit

Cat. Num. AAA25485

• Gene Names: VCAN; WGN; ERVR; GHAP; PG-M; WGN1; CSPG2
• Reactivity: Rat
• Synonyms: Versican; Rat Versican ELISA Kit; VCAN elisa kit

• Reactivity: Rat
• Specificity: No significant cross-reactivity or interference between Rat VCAN and analogues was observed.
• Sequence Length: 3396

• Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine
• Assay Type: Sandwich
• Detection Range: 3.12 ng/ml - 100 ng/ml.
• Sensitivity: 1.0 ng/ml.
• Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic applications! It is intended to be determinated VCAN concentrations in Rat serum, plasma and other body fluids. Using Purified Rat VCAN antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add VCAN and VCAN antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of VCAN in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Intra-assay Precision: Intra-assay CV (%) is less than 15%.
• Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]
• Preparation and Storage: Store all reagents at 2-8 degree C

Related Product Information for VCAN elisa kit

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only! It is intended to be determinated VCAN concentrations in Rat serum, plasma and other body fluids. Using Purified Rat VCAN antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add VCAN and VCAN antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of VCAN in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 21361116
• NCBI GeneID: 1462
• NCBI Accession #: NP_004376.2
• NCBI GenBank Nucleotide #: NM_004385.4
• UniProt Accession #: P13611; P20754; Q13010; Q13189; Q15123; Q9UCL9; Q9UNW5
• Molecular Weight: 372,820 Da
• NCBI Official Full Name: versican core protein isoform 1
• NCBI Official Synonym Full Names: versican
• NCBI Official Symbol: VCAN
• NCBI Official Synonym Symbols: WGN; ERVR; GHAP; PG-M; WGN1; CSPG2
• NCBI Protein Information: versican core protein; versican proteoglycan; large fibroblast proteoglycan; glial hyaluronate-binding protein; chondroitin sulfate proteoglycan 2; chondroitin sulfate proteoglycan core protein 2
• UniProt Protein Name: Versican core protein
• Protein Family: Versican core protein
• UniProt Gene Name: VCAN
• UniProt Synonym Gene Names: CSPG2; Chondroitin sulfate proteoglycan 2; GHAP
• UniProt Entry Name: CSPG2_HUMAN

NCBI Description

This gene is a member of the aggrecan/versican proteoglycan family. The protein encoded is a large chondroitin sulfate proteoglycan and is a major component of the extracellular matrix. This protein is involved in cell adhesion, proliferation, proliferation, migration and angiogenesis and plays a central role in tissue morphogenesis and maintenance. Mutations in this gene are the cause of Wagner syndrome type 1. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2009]

Uniprot Description

CSPG2: May play a role in intercellular signaling and in connecting cells with the extracellular matrix. May take part in the regulation of cell motility, growth and differentiation. Binds hyaluronic acid. Defects in VCAN are the cause of Wagner syndrome type 1 (WGN1). WGN is a dominantly inherited vitreoretinopathy characterized by an optically empty vitreous cavity with fibrillary condensations and a preretinal avascular membrane. Other optical features include progressive chorioretinal atrophy, perivascular sheating, subcapsular cataract and myopia. Systemic manifestations are absent in WGN. Belongs to the aggrecan/versican proteoglycan family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion; Secreted; Motility/polarity/chemotaxis; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 5q14.3

Cellular Component: extracellular matrix; extracellular space; proteinaceous extracellular matrix; lysosomal lumen; intracellular membrane-bound organelle; membrane; Golgi lumen; extracellular region

Molecular Function: protein binding; glycosaminoglycan binding; extracellular matrix structural constituent; hyaluronic acid binding; calcium ion binding; carbohydrate binding

Biological Process: extracellular matrix organization and biogenesis; central nervous system development; chondroitin sulfate biosynthetic process; glycosaminoglycan metabolic process; heart development; multicellular organismal development; cell recognition; pathogenesis; glial cell migration; dermatan sulfate biosynthetic process; osteoblast differentiation; chondroitin sulfate metabolic process; carbohydrate metabolic process; chondroitin sulfate catabolic process; cell adhesion; skeletal development

Disease: Wagner Vitreoretinopathy