Von Hippel-Lindau Protein Recombinant Protein | VBP1 recombinant protein

Von Hippel-Lindau Protein, Recombinant, Human (vHL)

Cat. Num. AAA638241

• Gene Names: VBP1; PFD3; PFDN3; VBP-1
• Purity: Highly Purified; 95% as determined by RP-HPLC, anion-exchange FPLC and/or reducing and non-reducing SDS-PAGE Silver Stained gel.
• Synonyms: Von Hippel-Lindau Protein; Recombinant; Human (vHL); VBP1 recombinant protein

• Host: E Coli
• Purity/Purification: Highly Purified; 95% as determined by RP-HPLC, anion-exchange FPLC and/or reducing and non-reducing SDS-PAGE Silver Stained gel.
• Form/Format: Supplied as a liquid in PBS, pH 7.4, 2mM EDTA, 1mM DTT..
• Sequence: MGSSHHHHHH SSGLVPRGSH MPRRAENWDE AEVGAEEAGV EEYGPEEDGG EESGAEESGPEESGPEELGA EEEMEAGRPR PVLRSVNSRE PSQVIFCNRS PRVVLPVWLN FDGEPQPYPT LPPGTGRRIH SYRGHLWLFR DAGTHDGLLV NQTELFVPSL NVDGQPIFAN ITLP

• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20 degree C. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for VBP1 recombinant protein

Von Hippel-Lindau disease(VHL) is a dominant inherited syndrome characterized by the predisposition to develop various kinds of benign and malignant tumors, including clear cell renal carcinomas, pheochromocytomas and hemangioblastomas of the central nervous system and retina. VHL syndrome is caused by germline mutation in the VHL tumor suppressor, and VHL tumors are associated with loss or mutation of the remaining wild-type allele. VHL has two domains: a roughly 100-residue NH2-terminal domain rich in beta sheet(beta-domain) and a smaller alpha-helical domain (alpha-domain), held together by two linkers and a polar interface. VHL protein is also involved in the degradation of hypoxia-inducible factor (HIF).

Recombinant Human VHL beta-domian is a single,non-glycosylated polypeptide chain containing 174aa and has a molecular mass of 19.2kD.

Product Categories/Family for VBP1 recombinant protein

Molecular Biology; MB-Disease Markers

NCBI and Uniprot Product Information

• NCBI GI #: 2738244
• NCBI GeneID: 7411
• UniProt Accession #: P61758; O55228; Q15765; Q5JT81; Q86X96; B2R8L5
• Molecular Weight: 19.2kD
• NCBI Official Full Name: von Hippel-Lindau binding protein
• NCBI Official Synonym Full Names: von Hippel-Lindau binding protein 1
• NCBI Official Symbol: VBP1
• NCBI Official Synonym Symbols: PFD3; PFDN3; VBP-1
• NCBI Protein Information: prefoldin subunit 3; HIBBJ46; prefoldin 3; VHL binding protein-1; VHL-binding protein 1; von Hippel-Lindau-binding protein 1
• UniProt Protein Name: Prefoldin subunit 3
• UniProt Gene Name: VBP1
• UniProt Synonym Gene Names: PFDN3; VBP-1
• UniProt Entry Name: PFD3_HUMAN

NCBI Description

The protein encoded by this gene interacts with the Von Hippel-Lindau protein to form an intracellular complex. Because it functions as a chaperone protein, it is suspected that it may play a role in the transport of the Von Hippel-Lindau protein from the perinuclear granules to the nucleus or cytoplasm. [provided by RefSeq, Jul 2008]

Uniprot Description

VBP1: Binds specifically to cytosolic chaperonin (c-CPN) and transfers target proteins to it. Binds to nascent polypeptide chain and promotes folding in an environment in which there are many competing pathways for nonnative proteins. Belongs to the prefoldin subunit alpha family.

Protein type: Chaperone

Chromosomal Location of Human Ortholog: Xq28

Cellular Component: intracellular membrane-bound organelle; cytoplasm; prefoldin complex; nucleus

Molecular Function: protein binding

Biological Process: 'de novo' posttranslational protein folding; cellular protein metabolic process; protein folding