Vav 3 Oncogene (VAV3) Recombinant Protein | VAV3 recombinant protein

Recombinant Vav 3 Oncogene (VAV3)

Cat. Num. AAA2011000

• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Vav 3 Oncogene (VAV3); Recombinant Vav 3 Oncogene (VAV3); VAV3 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSGSEF- HQPKCPEN DIRSCCLAEI KQTEEKYTET LESIEKYFMA PLKRFLTAAE FDSVFINIPE LVKLHRNLMQ EIHDSIVNKN DQNLYQVFIN YKERLVIYGQ YCSGVESAIS SLDYISKTKE DVKLKLEECS KRANNGKFTL RDLLVVPMQR VLKYHLLLQE LVKHTTDPTE KANLKLALDA MKDLAQYVNE VKRDNETLRE
• Sequence Length: 287

• Applicable Applications for VAV3 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Homo sapiens (Human)
• Expression System: Prokaryotic expression
• Residues: His183~Glu380 (Accession # Q9UKW4) with N-terminal His-Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 120432042
• NCBI GeneID: 10451
• NCBI Accession #: NP_001073343.1
• NCBI GenBank Nucleotide #: NM_001079874.1
• UniProt Accession #: Q9UKW4; O60498; O95230; Q9Y5X8; B1AMM0; B1APV5; B4E232; B7ZLR1; E9PQ97
• Molecular Weight: 24.7kDa
• NCBI Official Full Name: guanine nucleotide exchange factor VAV3 isoform 2
• NCBI Official Synonym Full Names: vav 3 guanine nucleotide exchange factor
• NCBI Official Symbol: VAV3
• NCBI Protein Information: guanine nucleotide exchange factor VAV3; VAV-3; vav 3 oncogene
• UniProt Protein Name: Guanine nucleotide exchange factor VAV3
• Protein Family: Guanine nucleotide exchange factor
• UniProt Gene Name: VAV3
• UniProt Synonym Gene Names: VAV-3
• UniProt Entry Name: VAV3_HUMAN

NCBI Description

This gene is a member of the VAV gene family. The VAV proteins are guanine nucleotide exchange factors (GEFs) for Rho family GTPases that activate pathways leading to actin cytoskeletal rearrangements and transcriptional alterations. This gene product acts as a GEF preferentially for RhoG, RhoA, and to a lesser extent, RAC1, and it associates maximally with the nucleotide-free states of these GTPases. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

VAV3: a guanine nucleotide exchange factor (GEF) that activates RhoA, RhoG and, to a lesser extent, Rac1. Binds physically to the nucleotide-free states of those GTPases. Plays an important role in angiogenesis. Its recruitment by activated EPHA2 is critical for EFNA1-induced RAC1 GTPase activation and vascular endothelial cell migration and assembly. May be important for integrin-mediated signaling, at least in some cell types. In osteoclasts, along with SYK tyrosine kinase, required for signaling through integrin alpha-v/beta-1 (ITAGV-ITGB1), a crucial event for osteoclast proper cytoskeleton organization and function. This signaling pathway involves RAC1, but not RHO, activation. Necessary for proper wound healing. In the course of wound healing, required for the phagocytotic cup formation preceding macrophage phagocytosis of apoptotic neutrophils. Responsible for integrin beta-2 (ITGB2)-mediated macrophage adhesion and, to a lesser extent, contributes to beta-3 (ITGB3)-mediated adhesion. Does not affect integrin beta-1 (ITGB1)-mediated adhesion. Interacts with the PH domain of APS. Interacts (via SH2 domains) with the phosphorylated form of EPHA2. Interacts with ROS1; constitutive interaction that mediates VAV3 phosphorylation. Down-regulated by EGF and TGF-beta. Four isoforms of the human protein are produced by alternative promoter. Isoform 1 and isoform 3 are widely expressed; both are expressed at very low levels in skeletal muscle. In keratinocytes, isoform 1 is less abundant than isoform 3. Isoform 3 is detected at very low levels, if any, in adrenal gland, bone marrow, spleen, fetal brain and spinal chord; in these tissues, isoform 1 is readily detectable.

Protein type: Motility/polarity/chemotaxis; GEFs, Rac/Rho; Actin-binding; GEFs; Nuclear receptor co-regulator; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 1p13.3

Cellular Component: plasma membrane; cytosol

Molecular Function: protein binding; SH3/SH2 adaptor activity; metal ion binding; guanyl-nucleotide exchange factor activity; Rac guanyl-nucleotide exchange factor activity; GTPase activator activity; epidermal growth factor receptor binding

Biological Process: response to drug; integrin-mediated signaling pathway; lamellipodium biogenesis; neutrophil chemotaxis; axon guidance; platelet activation; positive regulation of cell adhesion; nerve growth factor receptor signaling pathway; positive regulation of signal transduction; positive regulation of apoptosis; positive regulation of phosphoinositide 3-kinase activity; vesicle fusion; regulation of small GTPase mediated signal transduction; B cell receptor signaling pathway; small GTPase mediated signal transduction; ephrin receptor signaling pathway; positive regulation of B cell proliferation; innate immune response; angiogenesis; vascular endothelial growth factor receptor signaling pathway; blood coagulation; response to DNA damage stimulus