Vang Like Protein 1 (VANGL1) Recombinant Protein | VANGL1 recombinant protein

Recombinant Vang Like Protein 1 (VANGL1)

Cat. Num. AAA2010312

• Gene Names: Vangl1; Lpp2; Stb2; Stbm; Kitenin
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Vang Like Protein 1 (VANGL1); Recombinant Vang Like Protein 1 (VANGL1); VANGL1 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF-VD ALLFIHYLAI VLLELRQLQP MFTLQVVRST DGESRFYSLG HLSIQRAALV VLENYYKDFT IYNPNLLTAS KFRAAKHMAG LKVYNVDGPS NNATGQSRAM IAAAARRRDS SHNELYYEEA EHERRVKKRR ARLVVAVEEA FIHIQRLQAE EQQKSPGEVM DPREAAQAIF PSMARALQKY LRTTRQQHYH SMESILQHLA FCITNSMTPK AFLERYLSAG PTLQYDKDRW LSTQWRLISE EAVTNG
• Sequence Length: 526

• Applicable Applications for VANGL1 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Mus musculus (Mouse)
• Expression System: Prokaryotic expression
• Residues: Val229~Gly476 (Accession # Q80Z96) with two N-terminal Tags, His-tag and T7-tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 260099697
• NCBI GeneID: 229658
• NCBI Accession #: NP_808213.2
• NCBI GenBank Nucleotide #: NM_177545.5
• UniProt Accession #: Q80Z96; Q8QZT3; B2RRP5
• Molecular Weight: 32.4kDa
• NCBI Official Full Name: vang-like protein 1 isoform 1
• NCBI Official Synonym Full Names: vang-like 1 (van gogh, Drosophila)
• NCBI Official Symbol: Vangl1
• NCBI Official Synonym Symbols: Lpp2; Stb2; Stbm; Kitenin
• NCBI Protein Information: vang-like protein 1; strabismus; loop-tail protein 2; vang, van gogh-like 1; van Gogh-like protein 1; KAI1 COOH-terminal interacting tetraspanin
• UniProt Protein Name: Vang-like protein 1
• Protein Family: Vang-like protein
• UniProt Gene Name: Vangl1
• UniProt Synonym Gene Names: Lpp2
• UniProt Entry Name: VANG1_MOUSE

Uniprot Description

VANGL1: Defects in VANGL1 are a cause of neural tube defects (NTD). NTD are congenital malformations. The most common forms of NTD are described as open defects (including anencephaly and myelomeningocele, or spina bifida), which result from the failure of fusion in the cranial and spinal region of the neural tube, respectively. Other open dysraphisms (including myeloschisis, hemimyelomeningocele, and hemimyelocele) are sometimes associated with a Chiari type 2 malformation. A number of skin-covered (closed) NTD are categorized clinically depending on the presence of a subcutaneous mass (lipomyeloschisis, lipomyelomeningocele, meningocele, and myelocystocele) or the absence of such a mass (complex dysraphic states, including split cord malformations, dermal sinus, caudal regression, and segmental spinal dysgenesis). Defects in VANGL1 are a cause of sacral defect with anterior meningocele (SDAM). SDAM is a form of caudal dysgenesis. It is present at birth and becomes symptomatic later in life, usually because of obstructive labor in females, chronic constipation, or meningitis. Inheritance is autosomal dominant. Belongs to the Vang family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Adaptor/scaffold; Membrane protein, multi-pass; Motility/polarity/chemotaxis; Membrane protein, integral

Cellular Component: membrane; integral to membrane; plasma membrane; lateral plasma membrane

Molecular Function: protein binding

Biological Process: multicellular organismal development