Vesicle Associated Membrane Protein 2 (VAMP2) Recombinant Protein | VAMP2 recombinant protein

Recombinant Vesicle Associated Membrane Protein 2 (VAMP2)

Cat. Num. AAA2124618

• Gene Names: VAMP2; SYB2; VAMP-2
• Applications: SDS-Page, Western Blot
• Purity: >80%
• Synonyms: Vesicle Associated Membrane Protein 2 (VAMP2); Recombinant Vesicle Associated Membrane Protein 2 (VAMP2); Vesicle Associated Membrane Protein 2; SYB2; VAMP-2; Synaptobrevin 2; VAMP2 recombinant protein

• Host: E Coli
• Purity/Purification: >80%
• Form/Format: Freeze-dried powder; 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Sequence: Ser2~Thr116

• Applicable Applications for VAMP2 recombinant protein: Positive Control, Immunogen, SDS-PAGE, Western Blot (WB)
• Source: Prokaryotic expression
• Species: Human
• Tag: N-terminal His and GST Tag
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Isoelectric Point: 8.5
• Subcellular Location: Membrane
• Usage: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
• Preparation and Storage: Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months. Avoid repeated freeze/thaw cycles.; The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Sequence Information

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Gene Sequence

(Figure. Gene Sequencing (extract))

SDS-Page

(Figure. SDS-PAGE)

Product Categories/Family for VAMP2 recombinant protein

Neuro science; Recombinant proteins/RP

NCBI and Uniprot Product Information

• NCBI GI #: 288558837
• NCBI GeneID: 6844
• UniProt Accession #: P63027; P19065; Q9BUC2
• Molecular Weight: 13kDa
• NCBI Official Full Name: Vesicle-associated membrane protein 2
• NCBI Official Synonym Full Names: vesicle-associated membrane protein 2 (synaptobrevin 2)
• NCBI Official Symbol: VAMP2
• NCBI Official Synonym Symbols: SYB2; VAMP-2
• NCBI Protein Information: vesicle-associated membrane protein 2; synaptobrevin 2; synaptobrevin-2; vesicle associated membrane protein 2
• UniProt Protein Name: Vesicle-associated membrane protein 2
• Protein Family: Vesicle-associated membrane protein
• UniProt Gene Name: VAMP2
• UniProt Synonym Gene Names: SYB2; VAMP-2
• UniProt Entry Name: VAMP2_HUMAN

NCBI Description

The protein encoded by this gene is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family. Synaptobrevins/VAMPs, syntaxins, and the 25-kD synaptosomal-associated protein SNAP25 are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane. This gene is thought to participate in neurotransmitter release at a step between docking and fusion. The protein forms a stable complex with syntaxin, synaptosomal-associated protein, 25 kD, and synaptotagmin. It also forms a distinct complex with synaptophysin. It is a likely candidate gene for familial infantile myasthenia (FIMG) because of its map location and because it encodes a synaptic vesicle protein of the type that has been implicated in the pathogenesis of FIMG. [provided by RefSeq, Jul 2008]

Uniprot Description

VAMP2: a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family. Synaptobrevins/VAMPs, syntaxins, and the 25-kD synaptosomal-associated protein SNAP25 are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane. Thought to participate in neurotransmitter release at a step between docking and fusion. The protein forms a stable complex with syntaxin, synaptosomal-associated protein, 25 kD, and synaptotagmin. It also forms a distinct complex with synaptophysin.

Protein type: Motility/polarity/chemotaxis; Vesicle; Membrane protein, integral

Chromosomal Location of Human Ortholog: 17p13.1

Cellular Component: zymogen granule membrane; neuron projection; synaptic vesicle membrane; intracellular membrane-bound organelle; clathrin-coated vesicle; integral to plasma membrane; secretory granule membrane; trans-Golgi network; cytosol; secretory granule; SNARE complex; synaptic vesicle; membrane; perinuclear region of cytoplasm; plasma membrane; synapse; cytoplasmic vesicle; cell junction

Molecular Function: calmodulin binding; SNAP receptor activity; protein binding; SNARE binding; protein self-association; syntaxin binding; syntaxin-1 binding; phospholipid binding; calcium-dependent protein binding

Biological Process: exocytosis; natural killer cell degranulation; neurotransmitter secretion; neutrophil degranulation; pathogenesis; calcium ion-dependent exocytosis; vesicle fusion; regulation of exocytosis; vesicle-mediated transport; synaptic transmission; protein transport; synaptic vesicle exocytosis; cellular protein metabolic process; glutamate secretion; cellular response to insulin stimulus; Golgi to plasma membrane protein transport; response to glucose stimulus; energy reserve metabolic process; eosinophil degranulation; protein complex assembly; post-Golgi vesicle-mediated transport; regulation of insulin secretion