Ubiquitin Specific Peptidase 7 Recombinant Protein | USP7 recombinant protein

Recombinant Ubiquitin Specific Peptidase 7 (USP7)

Cat. Num. AAA2030457

• Gene Names: USP7; TEF1; HAUSP
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >95%
• Synonyms: Ubiquitin Specific Peptidase 7; Recombinant Ubiquitin Specific Peptidase 7 (USP7); USP7 recombinant protein

• Host: Host: E Coli; Source: Prokaryotic expression
• Purity/Purification: >95%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; EATFQFT VERFSRLSES VLSPPCFVRN LPWKIMVMPR FYPDRPHQKS VGFFLQCNAE SDSTSWSCHA QAVLKIINYR DDEKSFSRRI SHLFFHKEND WGFSNFMAWS EVTDPEKGFI DDDKVTFEVF VQADAPHGVA WDSKKHTGYV GLKNQGATCY MNSLLQTLFF TNQLRKAVYM MPTEGDDSSK SVPLALQRVF YELQHSDKPV GTKKLTKSFG WETLDSFMQH DVQELCRVLL DNVENKMKGT CVEGTIPKLF RGKMVSYIQC KEVDYRSDRR EDYYDIQLSI KGKKNIFESF VDYVAVEQLD GDNKYDAGEH GLQEAEKGVK FLTLPPVLHL QLMRFMYDPQ TDQNIKINDR FEFPEQLPLD EFLQKTDPKD PANYILHAVL VHSGDNHGGH YVVYLNPKGD GKWCKFDDDVVSRCTKEEAI EHNYGGHDDD LSVRHCTNAY MLVYIRE
• Sequence Length: 947

• Applicable Applications for USP7 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Predicted Molecular Mass: 56.3kDa
• Accurate Molecular Mass (KD): 56kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Tag: N-terminal His-Tag, T7 tag
• Organism Species: Homo sapiens (Human)
• Fragment: Glu54~Glu507
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 119605601
• NCBI GeneID: 7874
• Molecular Weight: – Da
• NCBI Official Full Name: ubiquitin specific peptidase 7 (herpes virus-associated), isoform CRA_b
• NCBI Official Synonym Full Names: ubiquitin specific peptidase 7 (herpes virus-associated)
• NCBI Official Symbol: USP7
• NCBI Official Synonym Symbols: TEF1; HAUSP
• NCBI Protein Information: ubiquitin carboxyl-terminal hydrolase 7
• UniProt Protein Name: Ubiquitin carboxyl-terminal hydrolase 7
• UniProt Gene Name: USP7
• UniProt Synonym Gene Names: HAUSP
• UniProt Entry Name: UBP7_HUMAN

Uniprot Description

USP7: an enzyme that removes ubiquitin from its protein substrates. Binds to the herpes virus protein VMW110 that may therefore modulate its substrate specificity or activity to stabilize viral proteins. Regulates MDM2 and has a dynamic role in the p53-MDM2 pathway. Is reported to deubiquitinate and stabilize p53.

Protein type: EC 3.4.19.12; Ubiquitin conjugating system; Ubiquitin-specific protease; Protease

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: nuclear body; PML body; cytosol; nucleus

Molecular Function: protein C-terminus binding; protein binding; p53 binding; ubiquitin protein ligase binding; cysteine-type endopeptidase activity; ubiquitin-specific protease activity; transcription factor binding

Biological Process: proteasomal ubiquitin-dependent protein catabolic process; protein deubiquitination; maintenance of DNA methylation; regulation of transcription factor activity; viral reproduction; inhibition of NF-kappaB transcription factor; multicellular organismal development; transcription-coupled nucleotide-excision repair; histone deubiquitination