Isopeptidase T Recombinant Protein | USP5 recombinant protein

Isopeptidase T protein (long form)

Cat. Num. AAA5304060

• Purity: Isopeptidase T protein was purified using a a proprietary chromatographic technique.
• Synonyms: Isopeptidase T; Isopeptidase T protein (long form); Ubiquitin-specific protease 5 protein; USP5 protein; USP5 recombinant protein

• Host: Human
• Purity/Purification: Isopeptidase T protein was purified using a a proprietary chromatographic technique.
• Form/Format: Supplied in 50 mM Tris, 10 Mmn beta-mercaptoethanol, pH 7.5.
• Sequence Length: 835

• Protein Type: Recombinant
• Biological Significance: Isopeptidase T is a ubiquitin-specific processing protease that specifically disassembles free K48-linked polyubiquitin chains into ubiquitin monomers. There are two human isopeptidase T isoforms; Isopeptidase T short form and Isopeptidase T long form which differ by an insertion of 23 amino acids in iso T-L. Isopeptidase T is the only UBP known to require zinc binding to be active.
• Expression System: E Coli
• Preparation and Storage: Store at -80 degree C

Related Product Information for USP5 recombinant protein

Purified recombinant Human Isopeptidase T protein (long form)

Product Categories/Family for USP5 recombinant protein

Proteases; Inhibitors; & Enzymes

NCBI and Uniprot Product Information

• NCBI GI #: 1732411
• Molecular Weight: 97 kDa
• NCBI Official Full Name: isopeptidase T
• UniProt Protein Name: Ubiquitin carboxyl-terminal hydrolase 5
• UniProt Gene Name: USP5
• UniProt Synonym Gene Names: ISOT
• UniProt Entry Name: UBP5_HUMAN

Uniprot Description

USP5: Cleaves linear and branched multiubiquitin polymers with a marked preference for branched polymers. Involved in unanchored 'Lys-48'-linked polyubiquitin disassembly. Binds linear and 'Lys- 63'-linked polyubiquitin with a lower affinity. Knock-down of USP5 causes the accumulation of p53/TP53 and an increase in p53/TP53 transcriptional activity because the unanchored polyubiquitin that accumulates is able to compete with ubiquitinated p53/TP53 but not with MDM2 for proteasomal recognition. Belongs to the peptidase C19 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin-specific protease; Protease; EC 3.4.19.12

Chromosomal Location of Human Ortholog: 12p13

Cellular Component: lysosome

Molecular Function: protein binding; omega peptidase activity; zinc ion binding; cysteine-type endopeptidase activity; ubiquitin-specific protease activity

Biological Process: ubiquitin-dependent protein catabolic process; positive regulation of proteasomal ubiquitin-dependent protein catabolic process