Mouse ubiquitin specific peptidase 5 (isopeptidase T) ELISA Kit | USP5 elisa kit

Mouse Ubiquitin carboxyl-terminal hydrolase 5, USP5 ELISA Kit

Cat. Num. AAA9338794

• Gene Names: Usp5; ISOT; Ucht; ISOT-1; AA407472
• Reactivity: Mouse
• Synonyms: ubiquitin specific peptidase 5 (isopeptidase T); Mouse Ubiquitin carboxyl-terminal hydrolase 5; USP5 ELISA Kit; Mouse Ubiquitin carboxyl-terminal hydrolase 5 (USP5) ELISA kit; ISOT; ubiquitin isopeptidase T; ubiquitin specific peptidase 5; ubiquitin specific protease 5 (isopeptidase T) ; ubiquitin-specific protease-5 (ubiquitin isopeptidase T) ; USP5 elisa kit

• Reactivity: Mouse

• Assay Type: Quantitative Sandwich
• Detection Range: 0.625ng/ml-20ng/ml
• Sensitivity: 0.1ng/ml
• Intra-assay Precision: Intra-assay CV (%) is less than 15%.
• Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]. All CV% should be compared by concentration, not compared by OD values.
• Preparation and Storage: Store all reagents at 2-8 degree C

Related Product Information for USP5 elisa kit

Background/Introduction: This Quantitative Sandwich ELISA kit is for lab reagent/research use only, not for drug, household, therapeutic or test applications! This kit is intended to be used for determine the level of USP5 (hereafter termed "analyte") in undiluted original Mouse serum, plasma or tissue homogenates samples. For other sample types please contact tech support to determine compatibility with this assay. This kit is not suitable for assaying non-biological sources of substances.

NCBI and Uniprot Product Information

• NCBI GI #: 7305619
• NCBI GeneID: 22225
• NCBI Accession #: NP_038728.1
• NCBI GenBank Nucleotide #: NM_013700.1
• UniProt Accession #: P56399
• Molecular Weight: 95,833 Da
• NCBI Official Full Name: ubiquitin carboxyl-terminal hydrolase 5
• NCBI Official Synonym Full Names: ubiquitin specific peptidase 5 (isopeptidase T)
• NCBI Official Symbol: Usp5
• NCBI Official Synonym Symbols: ISOT; Ucht; ISOT-1; AA407472
• NCBI Protein Information: ubiquitin carboxyl-terminal hydrolase 5; isopeptidase T; ubiquitin thioesterase 5; deubiquitinating enzyme 5; ubiquitin thiolesterase 5; ubiquitin specific protease 5; ubiquitin carboxy terminal hydrolase T; ubiquitin-specific-processing protease 5
• UniProt Protein Name: Ubiquitin carboxyl-terminal hydrolase 5
• UniProt Gene Name: Usp5
• UniProt Synonym Gene Names: Isot
• UniProt Entry Name: UBP5_MOUSE

Uniprot Description

USP5: Cleaves linear and branched multiubiquitin polymers with a marked preference for branched polymers. Involved in unanchored 'Lys-48'-linked polyubiquitin disassembly. Binds linear and 'Lys- 63'-linked polyubiquitin with a lower affinity. Knock-down of USP5 causes the accumulation of p53/TP53 and an increase in p53/TP53 transcriptional activity because the unanchored polyubiquitin that accumulates is able to compete with ubiquitinated p53/TP53 but not with MDM2 for proteasomal recognition. Belongs to the peptidase C19 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin-specific protease; Protease; EC 3.4.19.12

Cellular Component: lysosome

Molecular Function: peptidase activity; protein binding; omega peptidase activity; zinc ion binding; hydrolase activity; metal ion binding; cysteine-type endopeptidase activity; ubiquitin-specific protease activity; cysteine-type peptidase activity

Biological Process: ubiquitin-dependent protein catabolic process; proteasomal ubiquitin-dependent protein catabolic process; protein deubiquitination; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; proteolysis