Ubiquitin, Mutated Recombinant Protein | Usp2 recombinant protein

Ubiquitin, Mutated (Lys48 to Arg48), Recombinant, Human

Cat. Num. AAA637710

• Gene Names: Usp2; Ubp41; B930035K21Rik
• Purity: Highly Purified; 95% by SDS-PAGE commassie blue staining gel.
• Synonyms: Ubiquitin; Mutated; Mutated (Lys48 to Arg48); Recombinant; Human; Usp2 recombinant protein

• Host: E Coli
• Purity/Purification: Highly Purified; 95% by SDS-PAGE commassie blue staining gel.
• Form/Format: Supplied as a liquid in PBS, 5% glycerol.

• Preparation and Storage: Store vial at -20 degree C to -80 degree C. When stored at the recommended temperature, this protein is stable for 12 months. Please avoid freeze-thaw cycles.

Related Product Information for Usp2 recombinant protein

Ubiquitin is a small, evolutionarily conserved eukaryotic protein that can be attached to a wide variety of intracellular proteins including itself. Covalent attachment of ubiquitin to other proteins serves various functions, but its major role is to target cellular proteins for destruction. Cellular components that activate, transfer, remove, or simply recognize ubiquitin number in the hundreds, perhaps even in the thousands. In light of this complexity the ubiquitin pathway is ideal for a systems biology approach. Ubiquitin (Ub) plays a very important role in regulated non-lysosomal ATP dependent protein degradation. The protein to be degraded is conjugated to Ub and the ubiquinated protein is then selectively degraded by a 26S complex, multicatalytic cytosolic and nuclear protease termed proteasome. The Ub-proteasome proteolytic pathway, which is a complex process, is implicated to be of great importance for regulating numerous cellular processes.

Ubiquitin is a highly conserved 76 amino acid protein expressed in all eukariotes. Ub is found either in free form or conjugated to proteins as monomer or as chain of ubiquitin molecules. The most well characterized consequence of polyubiquitination is substrate degradation, while mono-ubiquitinated proteins are not degraded. Ubiquitin attachment to protein substrate is a complex process involving an ubiquitin activating enzyme (E1), an ubiquitin conjugating enzyme (E2) and an ubiquitin protein ligase (E3). The first ubiquitin moiety is transferred to the e-NH2 group of a Lys residue of the protein substrate to generate an isopeptide bond. In successive reactions, a poly ubiquitin chain is synthesized by processive transfer of additional activated moieties to Lys48 of the previously conjugated ubiquitin molecule. Ubiquitin K48R prevents the formation of poly ubiquitin chains via Lys48 linkages with mono ubiquitin molecules, avoiding the degradation of protein substrates. Recombinant human ubiquitin featuring a Lys 48 to Arg48 mutation, useful for the reduction of poly-Ub chain length and conjugation rates.

Product Categories/Family for Usp2 recombinant protein

Molecular Biology; MB-Ubiquitin

NCBI and Uniprot Product Information

• NCBI GI #: 114052502
• NCBI GeneID: 53376
• NCBI Accession #: NP_058088.2
• NCBI GenBank Nucleotide #: NM_016808.2
• UniProt Accession #: O88623; Q6X4T9; Q6X4U1; Q8VD74; Q9JJ87
• Molecular Weight: 68,845 Da
• NCBI Official Full Name: ubiquitin carboxyl-terminal hydrolase 2 isoform Usp2-69
• NCBI Official Synonym Full Names: ubiquitin specific peptidase 2
• NCBI Official Symbol: Usp2
• NCBI Official Synonym Symbols: Ubp41; B930035K21Rik
• NCBI Protein Information: ubiquitin carboxyl-terminal hydrolase 2; ubiquitin thioesterase 2; deubiquitinating enzyme 2; ubiquitin thiolesterase 2; ubiquitin specific protease 2; 41 kDa ubiquitin-specific protease; ubiquitin-specific-processing protease 2
• UniProt Protein Name: Ubiquitin carboxyl-terminal hydrolase 2
• Protein Family: Protein
• UniProt Gene Name: Usp2
• UniProt Synonym Gene Names: Ubp41
• UniProt Entry Name: UBP2_MOUSE

Uniprot Description

USP2: Hydrolase that deubiquitinates polyubiquitinated target proteins such as MDM2, MDM4 and CCND1. Isoform 1 and isoform 4 possess both ubiquitin-specific peptidase and isopeptidase activities. Deubiquitinates MDM2 without reversing MDM2-mediated p53/TP53 ubiquitination and thus indirectly promotes p53/TP53 degradation and limits p53 activity. Has no deubiquitinase activity against p53/TP53. Prevents MDM2-mediated degradation of MDM4. Plays a role in the G1/S cell-cycle progression in normal and cancer cells. Plays a role in the regulation of myogenic differentiation of embryonic muscle cells. Homooligomer. Found in trimeric complex with MDM2 and MDM4 and UPB2. Interacts with CCND1; the interaction is direct and promotes its stabilization by antagonizing ubiquitin-dependent degradation. Interacts (via N-terminus and C- terminus) with MDM2. Interacts with MDM4. Down-regulated by cisplatin. Expressed in mesangial cells of the kidney and in different types of glomerulonephritides. Cleavage is inhibited by ubiquitin in a dosage- dependent manner. Cleavage is blocked by ubiquitin aldehyde. Belongs to the peptidase C19 family. USP2 subfamily. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.19.12; Ubiquitin-specific protease; Protease

Cellular Component: perinuclear region of cytoplasm; cytoplasm; cell cortex; nucleus

Molecular Function: peptidase activity; identical protein binding; hydrolase activity; cysteine-type endopeptidase activity; metal ion binding; ubiquitin-specific protease activity; cysteine-type peptidase activity

Biological Process: ubiquitin-dependent protein catabolic process; proteasomal ubiquitin-dependent protein catabolic process; locomotor rhythm; muscle development; protein deubiquitination; regulation of transcription, DNA-dependent; rhythmic process; circadian regulation of gene expression; proteolysis; cell cycle; circadian behavior; entrainment of circadian clock by photoperiod