Ubiquitin, Mutated Recombinant Protein | Usp2 recombinant protein

Ubiquitin, Mutated (Gly76 to Ala76), Recombinant, Human

Cat. Num. AAA637225

• Gene Names: Usp2; Ubp41; B930035K21Rik
• Purity: Highly Purified; 95% by SDS-PAGE commassie blue staining gel.
• Synonyms: Ubiquitin; Mutated; Mutated (Gly76 to Ala76); Recombinant; Human; Usp2 recombinant protein

• Host: E Coli
• Purity/Purification: Highly Purified; 95% by SDS-PAGE commassie blue staining gel.
• Form/Format: Supplied as a liquid in PBS, 5% glycerol.

• Preparation and Storage: Store vial at -20 degree C to -80 degree C. When stored at the recommended temperature, this protein is stable for 12 months.

Related Product Information for Usp2 recombinant protein

The conserved 76 amino acid protein ubiquitin (Ub) regulates a host of intracellular processes through its enzymatic conjugation to other cellular proteins. Ubiquitination occurs through sequential steps catalyzed by activating (E1), conjugating (E2), and ligase (E3) enzymes. The final step results in the formation of an isopeptide bond between Ub's C-terminal glycine residue (G76) and a lysine residue of the target protein, although N-terminal ubiquitination is also known. Outcomes of this modification include destabilization of the conjugated protein, altered protein trafficking and functional modulation. After targeting the protein for specific localizations, ubiquitin is realised from the substrate by deubiquitinating enzymes. A mutant ubiquitin, having a Gly to Ala substitution at the C-terminus (G76A ubiquitin) supported several downstream reactions of the proteolytic pathway but inhibits the deubiquitination process. As consequence, the Ub derivative becomes irreversibly conjugated to protein, shifting the equilibrium between the bound and unbound form in the direction of conjugation, at the expense of the free form. Recombinant human ubiquitin featuring a Gly76 to Ala76 mutation that, inhibiting the ubiquitin hydrolases, prevents the removal of ubiquitin from proteinubiquitin conjugates. Ub G76A is expressed in Escherichia coli and purified by ion-exchange chromatography.

Product Categories/Family for Usp2 recombinant protein

Molecular Biology; MB-Ubiquitin

NCBI and Uniprot Product Information

• NCBI GI #: 114052502
• NCBI GeneID: 53376
• NCBI Accession #: NP_058088.2
• NCBI GenBank Nucleotide #: NM_016808.2
• UniProt Accession #: O88623; Q6X4T9; Q6X4U1; Q8VD74; Q9JJ87
• Molecular Weight: 68,845 Da
• NCBI Official Full Name: ubiquitin carboxyl-terminal hydrolase 2 isoform Usp2-69
• NCBI Official Synonym Full Names: ubiquitin specific peptidase 2
• NCBI Official Symbol: Usp2
• NCBI Official Synonym Symbols: Ubp41; B930035K21Rik
• NCBI Protein Information: ubiquitin carboxyl-terminal hydrolase 2; ubiquitin thioesterase 2; deubiquitinating enzyme 2; ubiquitin thiolesterase 2; ubiquitin specific protease 2; 41 kDa ubiquitin-specific protease; ubiquitin-specific-processing protease 2
• UniProt Protein Name: Ubiquitin carboxyl-terminal hydrolase 2
• Protein Family: Protein
• UniProt Gene Name: Usp2
• UniProt Synonym Gene Names: Ubp41
• UniProt Entry Name: UBP2_MOUSE

Uniprot Description

USP2: Hydrolase that deubiquitinates polyubiquitinated target proteins such as MDM2, MDM4 and CCND1. Isoform 1 and isoform 4 possess both ubiquitin-specific peptidase and isopeptidase activities. Deubiquitinates MDM2 without reversing MDM2-mediated p53/TP53 ubiquitination and thus indirectly promotes p53/TP53 degradation and limits p53 activity. Has no deubiquitinase activity against p53/TP53. Prevents MDM2-mediated degradation of MDM4. Plays a role in the G1/S cell-cycle progression in normal and cancer cells. Plays a role in the regulation of myogenic differentiation of embryonic muscle cells. Homooligomer. Found in trimeric complex with MDM2 and MDM4 and UPB2. Interacts with CCND1; the interaction is direct and promotes its stabilization by antagonizing ubiquitin-dependent degradation. Interacts (via N-terminus and C- terminus) with MDM2. Interacts with MDM4. Down-regulated by cisplatin. Expressed in mesangial cells of the kidney and in different types of glomerulonephritides. Cleavage is inhibited by ubiquitin in a dosage- dependent manner. Cleavage is blocked by ubiquitin aldehyde. Belongs to the peptidase C19 family. USP2 subfamily. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin-specific protease; Protease; EC 3.4.19.12

Cellular Component: perinuclear region of cytoplasm; cytoplasm; cell cortex; nucleus

Molecular Function: peptidase activity; identical protein binding; hydrolase activity; cysteine-type endopeptidase activity; metal ion binding; ubiquitin-specific protease activity; cysteine-type peptidase activity

Biological Process: ubiquitin-dependent protein catabolic process; proteasomal ubiquitin-dependent protein catabolic process; locomotor rhythm; muscle development; protein deubiquitination; regulation of transcription, DNA-dependent; rhythmic process; circadian regulation of gene expression; cell cycle; proteolysis; circadian behavior; entrainment of circadian clock by photoperiod