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Canine Ubiquitin carboxyl-terminal hydrolase 11 ELISA Kit | USP11 elisa kit

Canine Ubiquitin carboxyl-terminal hydrolase 11 (USP11) ELISA Kit

Gene Names
USP11; UHX1
Reactivity
Canine
Synonyms
Ubiquitin carboxyl-terminal hydrolase 11; Canine Ubiquitin carboxyl-terminal hydrolase 11 (USP11) ELISA Kit; RP4-659F15.2; UHX1; deubiquitinating enzyme 11|ubiquitin carboxyl-terminal hydrolase; X-linked|ubiquitin thiolesterase 11|ubiquitin-specific processing protease 11; USP11 elisa kit
Ordering
For Research Use Only!
Reactivity
Canine
Specificity
This assay has high sensitivity and excellent specificity for detection of Canine USP11. No significant cross-reactivity or interference between Canine USP11 and analogues was observed.
Sequence Length
963
Samples
Serum, Plasma, Other biological fluids
Assay Type
Sandwich
Sample Volume
1-200 uL
Precision
Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Detection Wavelength
450 nm
Preparation and Storage
Store at 2-8 degree C.
Related Product Information for USP11 elisa kit
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate USP11 in samples. An antibody specific for USP11 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyUSP11 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for USP11 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of USP11 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
109,817 Da
NCBI Official Full Name
ubiquitin carboxyl-terminal hydrolase 11
NCBI Official Synonym Full Names
ubiquitin specific peptidase 11
NCBI Official Symbol
USP11
NCBI Official Synonym Symbols
UHX1
NCBI Protein Information
ubiquitin carboxyl-terminal hydrolase 11
UniProt Protein Name
Ubiquitin carboxyl-terminal hydrolase 11
UniProt Gene Name
USP11
UniProt Synonym Gene Names
UHX1
UniProt Entry Name
UBP11_HUMAN

NCBI Description

Protein ubiquitination controls many intracellular processes, including cell cycle progression, transcriptional activation, and signal transduction. This dynamic process, involving ubiquitin conjugating enzymes and deubiquitinating enzymes, adds and removes ubiquitin. Deubiquitinating enzymes are cysteine proteases that specifically cleave ubiquitin from ubiquitin-conjugated protein substrates. This gene encodes a deubiquitinating enzyme which lies in a gene cluster on chromosome Xp11.23 [provided by RefSeq, Jul 2008]

Uniprot Description

USP11: Protease that can remove conjugated ubiquitin from target proteins and polyubiquitin chains. Inhibits the degradation of target proteins by the proteasome. Plays a role in the regulation of pathways leading to NF-kappa-B activation. Plays a role in the regulation of DNA repair after double-stranded DNA breaks. Belongs to the peptidase C19 family.

Protein type: Ubiquitin-specific protease; EC 3.4.19.12; Protease; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: Xp11.23

Cellular Component: cytoplasm; nucleus

Molecular Function: cysteine-type endopeptidase activity; protein binding; ubiquitin-specific protease activity

Biological Process: protein deubiquitination; ubiquitin-dependent protein catabolic process

Research Articles on USP11

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Product Notes

The Canine USP11 usp11 (Catalog #AAA285756) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA285756 ELISA Kit recognizes Canine USP11. It is sometimes possible for the material contained within the vial of "Ubiquitin carboxyl-terminal hydrolase 11, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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