Ubiquitin Specific Peptidase 1 Recombinant Protein | USP1 recombinant protein

Recombinant Ubiquitin Specific Peptidase 1 (USP1)

Cat. Num. AAA2033513

• Gene Names: USP1; UBP
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 90%
• Synonyms: Ubiquitin Specific Peptidase 1; Recombinant Ubiquitin Specific Peptidase 1 (USP1); UBP; Ubiquitin Carboxyl-Terminal Hydrolase 1; Ubiquitin Specific Protease 1; Deubiquitinating enzyme 1; Ubiquitin thioesterase 1; Ubiquitin-specific-processing protease 1; USP1 recombinant protein

• Host: E Coli
• Purity/Purification: > 90%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Sequence: MPGVIPSESN GLSRGSPSKK NRLSLKFFQK KETKRALDFT DSQEDEEKAS EYRGSEIDQV VPAAQSSPVS CEKRENLLPF VGLNNLGNTC YLNSILQVLY FCPGFKAGVK HLFNIISRKK EALKDDSIQK DKGSCKEDPL ASYELICSLQ SLIISVEQLQ ASFLLNPEKY TDELATQPRR LLNTLRELNP MYEGYLQH
• Sequence Length: 785

• Applicable Applications for USP1 recombinant protein: SDS-PAGE, Western (WB), ELISA (EIA), Immunoprecipitation (IP).
• Predicted Molecular Mass: 26.0kDa
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Endotoxin Level: <1.0EU per 1 ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Fragment: Met1~His198
• Organism Species: Rat
• Tag: two N-terminal Tags, His-tag and T7-tag
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 29791616
• NCBI GeneID: 7398
• Molecular Weight: 88,207 Da
• NCBI Official Full Name: Ubiquitin specific peptidase 1
• NCBI Official Synonym Full Names: ubiquitin specific peptidase 1
• NCBI Official Symbol: USP1
• NCBI Official Synonym Symbols: UBP
• NCBI Protein Information: ubiquitin carboxyl-terminal hydrolase 1
• UniProt Protein Name: Ubiquitin carboxyl-terminal hydrolase 1
• Protein Family: UDP-sugar pyrophosphorylase
• UniProt Gene Name: USP1
• UniProt Synonym Gene Names: hUBP
• UniProt Entry Name: UBP1_HUMAN

NCBI Description

This gene encodes a member of the ubiquitin-specific processing (UBP) family of proteases that is a deubiquitinating enzyme (DUB) with His and Cys domains. This protein is located in the cytoplasm and cleaves the ubiquitin moiety from ubiquitin-fused precursors and ubiquitinylated proteins. The protein specifically deubiquitinates a protein in the Fanconi anemia (FA) DNA repair pathway. Alternate transcriptional splice variants have been characterized. [provided by RefSeq, Jul 2008]

Uniprot Description

USP1: Negative regulator of DNA damage repair which specifically deubiquitinates monoubiquitinated FANCD2. Also involved in PCNA-mediated translesion synthesis (TLS) by deubiquitinating monoubiquitinated PCNA. Has almost no deubiquitinating activity by itself and requires the interaction with WDR48 to have a high activity. Interacts with FANCD2 and PCNA. Interacts with WDR48. Down-regulated following DNA damage. Belongs to the peptidase C19 family.

Protein type: EC 3.4.19.12; Ubiquitin-specific protease; Ubiquitin conjugating system; Protease

Chromosomal Location of Human Ortholog: 1p31.3

Cellular Component: nucleoplasm; nucleus

Molecular Function: cysteine-type endopeptidase activity; protein binding; ubiquitin-specific protease activity

Biological Process: bypass DNA synthesis; DNA damage response, detection of DNA damage; DNA repair; protein deubiquitination; regulation of DNA repair; response to UV; skeletal development; ubiquitin-dependent protein catabolic process