Rat Uroplakin 3A ELISA Kit | UPK3A elisa kit

Rat Uroplakin 3A ELISA Kit

Cat. Num. AAA49186

• Gene Names: UPK3A; UP3A; UPK3; UPIII; UPIIIA
• Reactivity: Rat
• Synonyms: Uroplakin 3A; Rat Uroplakin 3A ELISA Kit; UPK3A elisa kit

• Reactivity: Rat
• Specificity: No significant cross-reactivity or interference between Rat UPK3A and analogues was observed.
• Sequence Length: 166

• Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine
• Assay Type: Sandwich
• Detection Range: 0.5 ng/ml - 16 ng/ml.
• Sensitivity: 0.1 ng/ml.
• Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic applications! It is intended to be determinated UPK3A concentrations in Rat serum, plasma and other body fluids. Using Purified Rat UPK3A antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add UPK3A and UPK3A antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of UPK3A in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Intra-assay Precision: Intra-assay CV (%) is less than 15%.
• Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]
• Preparation and Storage: Store all reagents at 2-8 degree C

Related Product Information for UPK3A elisa kit

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only! It is intended to be determinated UPK3A concentrations in Rat serum, plasma and other body fluids. Using Purified Rat UPK3A antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add UPK3A and UPK3A antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of UPK3A in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 266456217
• NCBI GeneID: 7380
• NCBI Accession #: NP_001161046.1
• NCBI GenBank Nucleotide #: NM_001167574.1
• UniProt Accession #: O75631; O60261; Q32N05; Q5TII6; B0QY25
• Molecular Weight: 30,670 Da
• NCBI Official Full Name: uroplakin-3a isoform 2
• NCBI Official Synonym Full Names: uroplakin 3A
• NCBI Official Symbol: UPK3A
• NCBI Official Synonym Symbols: UP3A; UPK3; UPIII; UPIIIA
• NCBI Protein Information: uroplakin-3a; uroplakin 3; uroplakin III
• UniProt Protein Name: Uroplakin-3a
• Protein Family: Uroplakin
• UniProt Gene Name: UPK3A
• UniProt Synonym Gene Names: UPK3; UP3a; UPIII
• UniProt Entry Name: UPK3A_HUMAN

NCBI Description

This gene encodes a member of the uroplakin family, a group of transmembrane proteins that form complexes on the apical surface of the bladder epithelium. Mutations in this gene may be associated with renal adysplasia. Alternatively spliced transcript variants have been described.[provided by RefSeq, Nov 2009]

Uniprot Description

UPK3A: Component of the asymmetric unit membrane (AUM); a highly specialized biomembrane elaborated by terminally differentiated urothelial cells. May play an important role in AUM-cytoskeleton interaction in terminally differentiated urothelial cells. It also contributes to the formation of urothelial glycocalyx which may play an important role in preventing bacterial adherence. Defects in UPK3A are a cause of renal adysplasia (RADYS); also known as renal agenesis or renal aplasia. Renal agenesis refers to the absence of one (unilateral) or both (bilateral) kidneys at birth. Bilateral renal agenesis belongs to a group of perinatally lethal renal diseases, including severe bilateral renal dysplasia, unilateral renal agenesis with contralateral dysplasia and severe obstructive uropathy. Belongs to the uroplakin-3 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 22q13.31

Cellular Component: endoplasmic reticulum membrane; apical plasma membrane; integral to membrane

Biological Process: epithelial cell differentiation; urinary bladder development; water transport; urea transport; cell morphogenesis; sodium ion homeostasis; kidney development; potassium ion homeostasis

Disease: Renal Hypodysplasia/aplasia 1