Guinea Pig UL16 Binding protein 4 ELISA Kit | ULBP4 elisa kit

Guinea pig UL16 Binding protein 4 ELISA Kit

Cat. Num. AAA752932

• Gene Names: RAET1E; RL-4; LETAL; ULBP4; N2DL-4; NKG2DL4; RAET1E2; bA350J20.7
• Reactivity: Guinea Pig
• Synonyms: UL16 Binding protein 4; Guinea pig UL16 Binding protein 4 ELISA Kit; ULBP4 elisa kit

• Reactivity: Guinea Pig

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 0.5-10ng/mL
• Sensitivity: 0.1ng/mL
• Intended Uses: This ULBP4 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Guinea pig ULBP4. This ELISA kit for research use only, not for therapeutic applications
• !Intended Uses: This ULBP4 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Guinea pig ULBP4. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ULBP4 elisa kit

Principle of the Assay: ULBP4 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ULBP4 antibody and an ULBP4-HRP conjugate. The assay sample and buffer are incubated together with ULBP4-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ULBP4 concentration since ULBP4 from samples and ULBP4-HRP conjugate compete for the anti-ULBP4 antibody binding site. Since the number of sites is limited, as more sites are occupied by ULBP4 from the sample, fewer sites are left to bind ULBP4-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ULBP4 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for ULBP4 elisa kit

Immunology

NCBI and Uniprot Product Information

• NCBI GI #: 31322668
• NCBI GeneID: 135250
• Molecular Weight: 23,978 Da
• NCBI Official Full Name: ULBP4
• NCBI Official Synonym Full Names: retinoic acid early transcript 1E
• NCBI Official Symbol: RAET1E
• NCBI Official Synonym Symbols: RL-4; LETAL; ULBP4; N2DL-4; NKG2DL4; RAET1E2; bA350J20.7
• NCBI Protein Information: NKG2D ligand 4; RAE-1-like transcript 4; lymphocyte effector toxicity activation ligand
• UniProt Protein Name: NKG2D ligand 4
• UniProt Gene Name: RAET1E
• UniProt Synonym Gene Names: LETAL; N2DL4; ULBP4; UNQ1867/PRO4303; N2DL-4; NKG2DL4; RL-4
• UniProt Entry Name: N2DL4_HUMAN

NCBI Description

This gene belong to the RAET1 family, which consists of major histocompatibility complex (MHC) class I-related genes located in a cluster on chromosome 6q24.2-q25.3. This and RAET1G protein differ from other RAET1 proteins in that they have type I membrane-spanning sequences at their C termini rather than glycosylphosphatidylinositol anchor sequences. This protein functions as a ligand for NKG2D receptor, which is expressed on the surface of several types of immune cells, and is involved in innate and adaptive immune responses. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Aug 2011]

Uniprot Description

RAET1E: Ligand for the NKG2D receptor. Delivers activating signals to NK cells and promotes tumor immune surveillance by inducing the expansion of anti-tumor cytotoxic lymphocytes. Belongs to the MHC class I family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 6q25.1

Cellular Component: extracellular region; integral to membrane; plasma membrane

Molecular Function: peptide antigen binding; natural killer cell lectin-like receptor binding

Biological Process: regulation of immune response; antigen processing and presentation of peptide antigen via MHC class I; natural killer cell mediated cytotoxicity; T cell mediated cytotoxicity