Human NKG2D ligand 2 ELISA Kit | ULBP2 elisa kit

Human NKG2D ligand 2 ELISA Kit

Cat. Num. AAA761361

• Gene Names: ULBP2; N2DL2; RAET1H; NKG2DL2; ALCAN-alpha
• Reactivity: Human
• Synonyms: NKG2D ligand 2; Human NKG2D ligand 2 ELISA Kit; ULBP2/ALCAN-alpha/N2DL2/NKG2DL2/RAET1H/ALCAN-alpha/N2DL2/N2DL-2/NKG2D ligand 2/RAET1HNKG2DL2/retinoic acid early transcript 1 H/Retinoic acid early transcript 1H/UL16 binding protein 2/UL16-binding protein 2; ULBP2 elisa kit

• Reactivity: Human
• Sequence Length: 246

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 12.5-800IU/ml
• Sensitivity: 7.5IU/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for ULBP2 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human ULBP2 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human ULBP2 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human ULBP2 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human ULBP2 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 13376824
• NCBI GeneID: 80328
• NCBI Accession #: NP_079493.1
• NCBI GenBank Nucleotide #: NM_025217.3
• Molecular Weight: 27,368 Da
• NCBI Official Full Name: NKG2D ligand 2 preproprotein
• NCBI Official Synonym Full Names: UL16 binding protein 2
• NCBI Official Symbol: ULBP2
• NCBI Official Synonym Symbols: N2DL2; RAET1H; NKG2DL2; ALCAN-alpha
• NCBI Protein Information: NKG2D ligand 2
• UniProt Protein Name: NKG2D ligand 2
• Protein Family: NKG2D ligand
• UniProt Gene Name: ULBP2
• UniProt Synonym Gene Names: N2DL2; RAET1H; N2DL-2; NKG2DL2
• UniProt Entry Name: N2DL2_HUMAN

NCBI Description

This gene encodes a major histocompatibility complex (MHC) class I-related molecule that binds to the NKG2D receptor on natural killer (NK) cells to trigger release of multiple cytokines and chemokines that in turn contribute to the recruitment and activation of NK cells. The encoded protein undergoes further processing to generate the mature protein that is either anchored to membrane via a glycosylphosphatidylinositol moiety, or secreted. Many malignant cells secrete the encoded protein to evade immunosurveillance by NK cells. This gene is located in a cluster of multiple MHC class I-related genes on chromosome 6. [provided by RefSeq, Jul 2015]

Uniprot Description

ULBP2: Ligand for the NKG2D receptor, together with at least ULBP1 and ULBP3. ULBPs activate multiple signaling pathways in primary NK cells, resulting in the production of cytokines and chemokines. Binding of ULBPs ligands to NKG2D induces calcium mobilization and activation of the JAK2, STAT5, ERK and PI3K kinase/Akt signal transduction pathway. In CMV infected cells, interacts with soluble CMV glycoprotein UL16. The interaction with UL16 blocked the interaction with the NKG2D receptor, providing a mechanism by which CMV infected cells might escape the immune system. UL16 also causes ULBP2 to be retained in the ER and cis- Golgi apparatus so that it does not reach the cell surface. Belongs to the MHC class I family.

Protein type: Membrane protein, GPI anchor

Chromosomal Location of Human Ortholog: 6q25

Cellular Component: anchored to plasma membrane; cell surface; extracellular space

Molecular Function: antigen binding; natural killer cell lectin-like receptor binding

Biological Process: antigen processing and presentation; natural killer cell activation; natural killer cell mediated cytotoxicity