Typical Testing Data/Standard Curve (for reference only)
Mouse UL16 Binding protein 1 ELISA Kit | ULBP1 elisa kit
Mouse UL16 Binding protein 1 ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: UINS ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-UINS antibody and an UINS-HRP conjugate. The assay sample and buffer are incubated together with UINS-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the UINS concentration since UINS from samples and UINS-HRP conjugate compete for the anti-UINS antibody binding site. Since the number of sites is limited, as more sites are occupied by UINS from the sample, fewer sites are left to bind UINS-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The UINS concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
Uniprot Description
ULBP1: Ligand for the NKG2D receptor, together with at least ULBP2 and ULBP3. ULBPs activate multiple signaling pathways in primary NK cells, resulting in the production of cytokines and chemokines. Binding of ULBPs ligands to NKG2D induces calcium mobilization and activation of the JAK2, STAT5, ERK and PI3K kinase/Akt signal transduction pathway. In CMV infected cells, interacts with soluble CMV glycoprotein UL16. The interaction with UL16 blocked the interaction with the NKG2D receptor, providing a mechanism by which CMV infected cells might escape the immune system. UL16 also causes ULBP1 to be retained in the ER and cis- Golgi apparatus so that it does not reach the cell surface. Belongs to the MHC class I family.
Protein type: Membrane protein, GPI anchor; Cell surface; Apoptosis
Chromosomal Location of Human Ortholog: 6q25
Cellular Component: anchored to plasma membrane; endoplasmic reticulum; plasma membrane
Molecular Function: peptide antigen binding; natural killer cell lectin-like receptor binding
Biological Process: antigen processing and presentation of peptide antigen via MHC class I; regulation of immune response; natural killer cell mediated cytotoxicity; natural killer cell activation
Research Articles on ULBP1
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Product Notes
The Mouse ULBP1 ulbp1 (Catalog #AAA745392) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA745392 ELISA Kit recognizes Mouse ULBP1. It is sometimes possible for the material contained within the vial of "UL16 Binding protein 1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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