Cytomegalovirus pp150 (UL32) Recombinant Protein | CMV pp150 recombinant protein
Cytomegalovirus pp150 (UL32) Recombinant
Purity
>95%, as determined by SDS-PAGE.
Synonyms
Cytomegalovirus pp150 (UL32); Cytomegalovirus pp150 (UL32) Recombinant; cmv; pp150; ul32; CMV pp150 recombinant protein
Host
E Coli
Purity/Purification
>95%, as determined by SDS-PAGE.
Form/Format
Lyophilized from 25mM Tris-Hcl pH 7.2, 1mM EDTA and 50% glycerol.
Sequence Length
1048
Application Notes
CMV pp150 antigen is suitable for ELISA and Western blots, excellent antigen for detection of CMV with minimal specificity problems.
Host Note
DNA sequence encoding immunodominant fragment of Cytomegalovirus 150kDa structural phosphoprotein was expressed in Escherichia Coli.
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Molecular Weight Note
Recombinant CMV pp150 (UL32) encodes the immunodominant sequences of the Cytomegalovirus matrix phosphoprotein. This molecule has a calculated mass of approximately 22kDa.
Recombinant CMV pp150 contains an N terminal GST purification tag and migrates as an approximately 52 kDa protein under non-reducing conditions and reducing conditions in SDS-PAGE.
Recombinant CMV pp150 contains an N terminal GST purification tag and migrates as an approximately 52 kDa protein under non-reducing conditions and reducing conditions in SDS-PAGE.
Preparation and Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20 degree C.
Upon reconstitution, this protein can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity.
Avoid repeated freeze/thaw cycles.
Upon reconstitution, this protein can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity.
Avoid repeated freeze/thaw cycles.
Related Product Information for CMV pp150 recombinant protein
The basic phosphoprotein 150 (pp150), the product of UL32 (unique long domain 32) gene of human cytomegalovirus (CMV), is an abundant component of the viral tegument and a target of human leukocyte antigen (HLA) -restricted cytotoxic T cells (CTLs) after infection.
Product Categories/Family for CMV pp150 recombinant protein
NCBI and Uniprot Product Information
Uniprot Description
Function: Participates in the last steps of viral maturation and release. Associates with nuclear capsids prior to DNA encapsidation and later preserves the integrity of nucleocapsids through secondary envelopment at the assembly compartment. Ref.9 Ref.10
Subunit structure: Interacts with host BICD1. Ref.11
Subcellular location: Virion tegument. Host cytoplasm. Host nucleus. Note: Found initially localized in the host nucleus, associated either with the nuclear membrane or with viral assembly regions, and later in the host cytoplasm. Ref.8
Post-translational modification: Phosphorylated at multiple sites.
Sequence similarities: Belongs to the herpesviridae large structural phosphoprotein family.
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Product Notes
The CMV pp150 ul32 (Catalog #AAA553087) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. CMV pp150 antigen is suitable for ELISA and Western blots, excellent antigen for detection of CMV with minimal specificity problems. . Researchers should empirically determine the suitability of the CMV pp150 ul32 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cytomegalovirus pp150 (UL32), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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