BRCA1-A complex subunit RAP80 (UIMC1) Recombinant Protein | UIMC1 recombinant protein

Recombinant Human BRCA1-A complex subunit RAP80 (UIMC1)

Cat. Num. AAA1361722

• Gene Names: UIMC1; RAP80; X2HRIP110
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: BRCA1-A complex subunit RAP80 (UIMC1); Recombinant Human BRCA1-A complex subunit RAP80 (UIMC1); UIMC1 recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Positions: 1-719, Full length protein
• Sequence: MPRRKKKVKEVSESRNLEKKDVETTSSVSVKRKRRLEDAFIVISDSDGEEPKEENGLQKTKTKQSNRAKCLAKRKIAQMTEEEQFALALKMSEQEAREVNSQEEEEEELLRKAIAESLNSCRPSDASATRSRPLATGPSSQSHQEKTTDSGLTEGIWQLVPPSLFKGSHISQGNEAEEREEPWDHTEKTEEEPVSGSSGSWDQSSQPVFENVNVKSFDRCTGHSAEHTQCGKPQESTGRGSAFLKAVQGSGDTSRHCLPTLADAKGLQDTGGTVNYFWGIPFCPDGVDPNQYTKVILCQLEVYQKSLKMAQRQLLNKKGFGEPVLPRPPSLIQNECGQGEQASEKNECISEDMGDEDKEERQESRASDWHSKTKDFQESSIKSLKEKLLLEEEPTTSHGQSSQGIVEETSEEGNSVPASQSVAALTSKRSLVLMPESSAEEITVCPETQLSSSETFDLEREVSPGSRDILDGVRIIMADKEVGNKEDAEKEVAISTFSSSNQVSCPLCDQCFPPTKIERHAMYCNGLMEEDTVLTRRQKEAKTKSDSGTAAQTSLDIDKNEKCYLCKSLVPFREYQCHVDSCLQLAKADQGDGPEGSGRACSTVEGKWQQRLKNPKEKGHSEGRLLSFLEQSEHKTSDADIKSSETGAFRVPSPGMEEAGCSREMQSSFTRRDLNESPVKSFVSISEATDCLVDFKKQVTVQPGSRTRTKAGRGRRRKF
• Sequence Length: 719

• Species: Human
• Preparation and Storage: Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.

NCBI and Uniprot Product Information

• NCBI GI #: 313151224
• NCBI GeneID: 51720
• NCBI Accession #: NP_001186226.1
• NCBI GenBank Nucleotide #: NM_001199297.2
• UniProt Accession #: Q96RL1; Q5XKQ1; Q7Z3W7; Q8N5B9; Q9BZR1; Q9BZR5; Q9UHX7; A8MSA1; B3KMZ1; B4E3N2
• Molecular Weight: 17,412 Da
• NCBI Official Full Name: BRCA1-A complex subunit RAP80 isoform 1
• NCBI Official Synonym Full Names: ubiquitin interaction motif containing 1
• NCBI Official Symbol: UIMC1
• NCBI Official Synonym Symbols: RAP80; X2HRIP110
• NCBI Protein Information: BRCA1-A complex subunit RAP80
• UniProt Protein Name: BRCA1-A complex subunit RAP80
• Protein Family: BRCA1-A complex
• UniProt Gene Name: UIMC1
• UniProt Synonym Gene Names: RAP80; RXRIP110

NCBI Description

This gene encodes a nuclear protein that interacts with Brca1 (breast cancer 1) in a complex to recognize and repair DNA lesions. This protein binds ubiquitinated lysine 63 of histone H2A and H2AX. This protein may also function as a repressor of transcription. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2015]

Uniprot Description

Ubiquitin-binding protein (PubMed:24627472). Specifically recognizes and binds 'Lys-63'-linked ubiquitin (PubMed:19328070, Ref. 37). Plays a central role in the BRCA1-A complex by specifically binding 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). The BRCA1-A complex also possesses deubiquitinase activity that specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX. Also weakly binds monoubiquitin but with much less affinity than 'Lys-63'-linked ubiquitin. May interact with monoubiquitinated histones H2A and H2B; the relevance of such results is however unclear in vivo. Does not bind Lys-48'-linked ubiquitin. May indirectly act as a transcriptional repressor by inhibiting the interaction of NR6A1 with the corepressor NCOR1.