Ubiquitin Conjugating Enzyme E2B Recombinant Protein | UBE2B recombinant protein

Recombinant Human Ubiquitin Conjugating Enzyme E2B

Cat. Num. AAA142481

• Gene Names: UBE2B; HR6B; UBC2; HHR6B; RAD6B; E2-17kDa
• Purity: Greater than 95.0% as determined by(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
• Synonyms: Ubiquitin Conjugating Enzyme E2B; Recombinant Human Ubiquitin Conjugating Enzyme E2B; UBE2B Human; Ubiquitin Conjugating Enzyme E2B Human Recombinant; Ubiquitin-conjugating enzyme E2 B; EC 6.3.2.19; Ubiquitin-protein ligase B; Ubiquitin carrier protein B; HR6B; hHR6B; E2-17 kDa UBC2; HHR6B; RAD6B; E2-17kDa; UBE2B; UBE2B recombinant protein

• Host: E Coli
• Purity/Purification: Greater than 95.0% as determined by(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
• Form/Format: Lyophilized from a 0.2um filtered concentrated (1 mg/ml) solution in 1X PBS and 1mM DTT, pH 7.5.; Sterile Filtered white lyophilized powder.
• Sequence: MHHHHHHAMGQLRSMSTPARRRLMRDFKRLQEDPPVGVSGAPSENNIMQWNAVIFGPEGTPFEDGTFKLVIEFSEEYPNKPPTVRFLSKMFHPNVYADGSICLDILQNRWSPTYDVSSILTSIQSLLDEPNPNSPANSQAAQLYQENKREYEKRVSAIVEQSWNDS
• Sequence Length: 152

• Solubility: It is recommended to reconstitute the lyophilized UBE2B in sterile water not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
• Preparation and Storage: Lyophilized UBE2B although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution UBE2B should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

Related Product Information for UBE2B recombinant protein

Description: Ubiquitin Conjugating Enzyme E2B Human Recombinant produced in E Coli is a 19 kDa protein containing 166 amino acids.The UE2B protein contains 6xHis tag and is purified by proprietary chromatographic techniques.

Introduction: This E2 enzyme encodes for the human homolog of the yeast DNA repair gene RAD6, which is induced by DNA damaging agents. UBE2B can conjugate ubiquitin to histone H2A in an E3- independent manner in vitro, and is essential for the multi-ubiquitination and degradation of N-end rule substrates. Additionally, UBE2B may have a role in sepsis-induced muscle protein proteolysis and cancer-induced cachexia.

Product Categories/Family for UBE2B recombinant protein

Enzymes; Ubiquitin Conjugating Enzyme

NCBI and Uniprot Product Information

• NCBI GI #: 4507771
• NCBI GeneID: 7320
• NCBI Accession #: NP_003328.1
• NCBI GenBank Nucleotide #: NM_003337.3
• UniProt Accession #: P63146; P23567; Q4PJ15; Q9D0J6; B2R503; D3DQA2
• Molecular Weight: 17,312 Da
• NCBI Official Full Name: ubiquitin-conjugating enzyme E2 B
• NCBI Official Synonym Full Names: ubiquitin-conjugating enzyme E2B
• NCBI Official Symbol: UBE2B
• NCBI Official Synonym Symbols: HR6B; UBC2; HHR6B; RAD6B; E2-17kDa
• NCBI Protein Information: ubiquitin-conjugating enzyme E2 B; E2 protein; RAD6 homolog B; ubiquitin carrier protein B; ubiquitin-conjugating enzyme E2-17 kDa; ubiquitin-conjugating enzyme E2B (RAD6 homolog); ubiquitin-protein ligase B
• UniProt Protein Name: Ubiquitin-conjugating enzyme E2 B
• Protein Family: Ubiquitin-conjugating enzyme
• UniProt Gene Name: UBE2B
• UniProt Synonym Gene Names: RAD6B; HR6B; hHR6B
• UniProt Entry Name: UBE2B_HUMAN

NCBI Description

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for post-replicative DNA damage repair. Its protein sequence is 100% identical to the mouse, rat, and rabbit homologs, which indicates that this enzyme is highly conserved in eukaryotic evolution. [provided by RefSeq, Jul 2008]

Uniprot Description

UBE2B: a ubiquitin-protein ligase, a member of the ubiquitin-conjugating enzyme family. Homologous to the yeast DNA repair gene RAD6. Interacts with RAD18. Required for postreplication repair of UV-damaged DNA.

Protein type: Ubiquitin ligase; EC 6.3.2.19; Ubiquitin conjugating system; Ligase

Chromosomal Location of Human Ortholog: 5q31.1

Cellular Component: XY body; cytoplasm; nuclear chromatin; plasma membrane; replication fork; chromatin; nucleus

Molecular Function: protein binding; small conjugating protein ligase activity; ubiquitin protein ligase binding; ubiquitin-protein ligase activity; ATP binding; ligase activity

Biological Process: ubiquitin-dependent protein catabolic process; response to drug; protein monoubiquitination; proteasomal ubiquitin-dependent protein catabolic process; protein autoubiquitination; protein polyubiquitination; protein stabilization; in utero embryonic development; postreplication repair; protein ubiquitination; Wnt receptor signaling pathway through beta-catenin; DNA repair; negative regulation of histone phosphorylation; cellular response to insulin stimulus; sperm axoneme assembly; response to hypoxia; maintenance of chromatin silencing; spermatogenesis; response to DNA damage stimulus; chiasma formation; histone H2A ubiquitination; response to UV; negative regulation of apoptosis