Ubiquitin-conjugating enzyme E2 35 (UBC35) Recombinant Protein | UBC35 recombinant protein

Recombinant Arabidopsis thaliana Ubiquitin-conjugating enzyme E2 35 (UBC35)

Cat. Num. AAA1424600

• Gene Names: UBC35; F9K20.8; F9K20_8; UBC13A; UBIQUITIN CONJUGATING ENZYME 13A; ubiquitin-conjugating enzyme 35
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: Ubiquitin-conjugating enzyme E2 35 (UBC35); Recombinant Arabidopsis thaliana Ubiquitin-conjugating enzyme E2 35 (UBC35); UBC35 recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Positions: 1-153, Full length protein
• Sequence: MANSNLPRRIIKETQRLLSEPAPGISASPSEDNMRYFNVMILGPTQSPYEGGVFKLELFLPEEYPMAAPKVRFLTKIYHPNIDKLGRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLSENIAKHWKSNEAEAVDTAKEWTRLYASGA
• Sequence Length: 153

• Species: Arabidopsis thaliana (Mouse-ear cress)
• Preparation and Storage: Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.

NCBI and Uniprot Product Information

• NCBI GI #: 79321461
• NCBI GeneID: 844224
• NCBI Accession #: NP_001031298.1
• NCBI GenBank Nucleotide #: NM_001036221.3
• UniProt Accession #: Q94A97; Q2V4C1; Q9ZVA6
• Molecular Weight: 12,851 Da
• NCBI Official Full Name: ubiquitin-conjugating enzyme 35
• NCBI Official Symbol: UBC35
• NCBI Official Synonym Symbols: F9K20.8; F9K20_8; UBC13A; UBIQUITIN CONJUGATING ENZYME 13A; ubiquitin-conjugating enzyme 35
• NCBI Protein Information: ubiquitin-conjugating enzyme 35
• UniProt Protein Name: Ubiquitin-conjugating enzyme E2 35
• Protein Family: Ubiquitin-conjugating enzyme
• UniProt Gene Name: UBC35
• UniProt Synonym Gene Names: UBC13A; UBG13A

NCBI Description

UBC35/UBC13A encodes a protein that may play a role in DNA damage responses and error-free post-replicative DNA repair by participating in lysine-63-based polyubiquitination reactions. UBC35/UBC13A can form diubiquitin and triubiquitin chains in combination with MMZ1,2,3,4/(UEV1A,B,C,D) in vitro. It can also functionally complement an mms2 ubc13 mutation in budding yeast by increasing the double mutant's viability in the presence of the DNA damaging agent MMS, when it is co-expressed with MMZ / UEV1 genes. A wild type phenotype is restored with MMZ3/UEV1C and MMZ4/UEV1D, but only partial complementation is achieved with MMZ1/UEV1A or MMZ2/UEV1B.

Uniprot Description

Catalyzes the synthesis of non-canonical poly-ubiquitin chains that are linked through 'Lys-63'. This type of poly-ubiquitination does not lead to protein degradation by the proteasome. Mediates transcriptional activation of target genes. Required for postreplication repair of UV-damaged DNA and for adapting root developmental programs to suboptimal availability of iron.