Sts1 Recombinant Protein | UBASH3B recombinant protein

Sts1 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8121561

• Gene Names: UBASH3B; p70; STS1; STS-1; TULA2; TULA-2
• Purity: >95% as determined by SDS-PAGE
• Synonyms: Sts1; Sts1 Protein; Human; Recombinant (His Tag); Human UBASH3B/Sts1/TULA-2 Protein (His Tag); p70 Protein; STS-1 Protein; STS1 Protein; TULA-2 Protein; TULA2 Protein; ubiquitin associated and SH3 domain containing B; UBASH3B recombinant protein

• Host: E Coli
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Gln382-Glu649

• Species: Human
• Predicted N Terminal: His
• Tag: N-His
• Protein Construction: A DNA sequence encoding the mature form of human UBASH3B(Q8TF42)(Gln382-Glu649) was expressed with a polyhistide tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for UBASH3B recombinant protein

Background: UBASH3B contains a ubiquitin associated domain at the N-terminus, an SH3 domain, and a C-terminal domain with similarities to the catalytic motif of phosphoglycerate mutase. UBASH3B was found to inhibit endocytosis of epidermal growth factor receptor (EGFR) and platelet-derived growth factor receptor. UBASH3B interferes with CBL-mediated down-regulation and degradation of receptor-type tyrosine kinases. It promotes accumulation of activated target receptors, such as T-cell receptors and EGFR, on the cell surface. UBASH3B exhibits tyrosine phosphatase activity toward several substrates including EGFR, FAK, SYK, and ZAP7. Down-regulates proteins that are dually modified by both protein tyrosine phosphorylation and ubiquitination.

Product Categories/Family for UBASH3B recombinant protein

Adaptor Proteins Phosphatases and Regulators

References

Maruyama K, et al. (1994) Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides. Gene. 138(1-2):171-4.Carpino N, et al. (2002) Identification, cDNA cloning, and targeted deletion of p70, a novel, ubiquitously expressed SH3 domain-containing protein. Mol Cell Biol. 22(21):7491-500.Nagase T, et al. (2002) Prediction of the coding sequences of unidentified human genes. XXII. The complete sequences of 50 new cDNA clones which code for large proteins. DNA Res. 8(6):319-27.

NCBI and Uniprot Product Information

• NCBI GI #: 110287974
• NCBI GeneID: 84959
• UniProt Accession #: Q8TF42; Q53GT5; Q53GT8; Q8NBV7; Q96IG9; Q96NZ2
• Molecular Weight: 649
• NCBI Official Full Name: Ubiquitin-associated and SH3 domain-containing protein B
• NCBI Official Synonym Full Names: ubiquitin associated and SH3 domain containing B
• NCBI Official Symbol: UBASH3B
• NCBI Official Synonym Symbols: p70; STS1; STS-1; TULA2; TULA-2
• NCBI Protein Information: ubiquitin-associated and SH3 domain-containing protein B; T-cell ubiquitin ligand 2; cbl-interacting protein p70; Cbl-interacting protein Sts-1; nm23-phosphorylated unknown substrate; tyrosine-protein phosphatase STS1/TULA2; suppressor of T-cell receptor
• UniProt Protein Name: Ubiquitin-associated and SH3 domain-containing protein B
• Protein Family: STS14 protein
• UniProt Gene Name: UBASH3B
• UniProt Synonym Gene Names: KIAA1959; STS1; STS-1; TULA-2
• UniProt Entry Name: UBS3B_HUMAN

NCBI Description

This gene encodes a protein that contains a ubiquitin associated domain at the N-terminus, an SH3 domain, and a C-terminal domain with similarities to the catalytic motif of phosphoglycerate mutase. The encoded protein was found to inhibit endocytosis of epidermal growth factor receptor (EGFR) and platelet-derived growth factor receptor. [provided by RefSeq, Jul 2008]

Uniprot Description

STS-1: Interferes with CBL-mediated down-regulation and degradation of receptor-type tyrosine kinases. Promotes accumulation of activated target receptors, such as T-cell receptors and EGFR, on the cell surface. Exhibits tyrosine phosphatase activity toward several substrates including EGFR, FAK, SYK, and ZAP70. Down-regulates proteins that are dually modified by both protein tyrosine phosphorylation and ubiquitination.

Protein type: EC 3.1.3.48; Protein phosphatase, tyrosine (non-receptor)

Chromosomal Location of Human Ortholog: 11q24.1

Cellular Component: cytoplasm; nucleus

Molecular Function: protein binding; protein tyrosine phosphatase activity

Biological Process: negative regulation of protein kinase activity; regulation of release of sequestered calcium ion into cytosol