Sheep Urotensin II ELISA Kit | U-II elisa kit

Sheep Urotensin II ELISA Kit

Cat. Num. AAA740201

• Gene Names: Uts2; Ucn2
• Reactivity: Sheep
• Synonyms: Urotensin II; Sheep Urotensin II ELISA Kit; U-II elisa kit

• Reactivity: Sheep
• Sequence Length: 123

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 1.0 ng/mL.
• Intended Uses: This U-II ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Sheep U-II. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for U-II elisa kit

Principle of the Assay: U-II ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-U-II antibody and an U-II-HRP conjugate. The assay sample and buffer are incubated together with U-II-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the U-II concentration since U-II from samples and U-II-HRP conjugate compete for the anti-U-II antibody binding site. Since the number of sites is limited, as more sites are occupied by U-II from the sample, fewer sites are left to bind U-II-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The U-II concentration in each sample is interpolated from this standard curve.

Product Categories/Family for U-II elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 9507227
• NCBI GeneID: 29180
• NCBI Accession #: NP_062033.1
• NCBI GenBank Nucleotide #: NM_019160.2
• UniProt Accession #: Q9QZQ4
• Molecular Weight: 13,614 Da
• NCBI Official Full Name: urotensin-2
• NCBI Official Synonym Full Names: urotensin 2
• NCBI Official Symbol: Uts2
• NCBI Official Synonym Symbols: Ucn2
• NCBI Protein Information: urotensin-2
• UniProt Protein Name: Urotensin-2
• Protein Family: Urotensin
• UniProt Gene Name: Uts2
• UniProt Synonym Gene Names: U-II; UII
• UniProt Entry Name: UTS2_RAT

NCBI Description

a cyclic neuropeptide; may be involved in the control of neuromuscular functions [RGD, Feb 2006]

Uniprot Description

Highly potent vasoconstrictor.