Human Tubulin Alpha 3c ELISA Kit | TUBa3C elisa kit

Human Tubulin Alpha 3c ELISA Kit

Cat. Num. AAA733232

• Gene Names: TUBA3D; H2-ALPHA
• Reactivity: Human
• Synonyms: Tubulin Alpha 3c; Human Tubulin Alpha 3c ELISA Kit; TUBa3C elisa kit

• Reactivity: Human

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 100-2500pg/mL
• Sensitivity: 1.0pg/mL
• Intended Uses: This TUBa3C ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human TUBa3C. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TUBa3C elisa kit

Principle of the Assay: TUBa3C ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for TUBa3C. Standards or samples are then added to the microtiter plate wells and TUBa3C if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of TUBa3C present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for TUBa3C are added to each well to "sandwich" the TUBa3C immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain TUBa3C and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TUBa3C concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 156564363
• NCBI GeneID: 113457
• NCBI Accession #: NP_525125.2
• NCBI GenBank Nucleotide #: NM_080386.3
• Molecular Weight: 46,112 Da
• NCBI Official Full Name: tubulin alpha-3C/D chain
• NCBI Official Synonym Full Names: tubulin, alpha 3d
• NCBI Official Symbol: TUBA3D
• NCBI Official Synonym Symbols: H2-ALPHA
• NCBI Protein Information: tubulin alpha-3C/D chain; tubulin alpha-2 chain; alpha-tubulin isotype H2-alpha
• UniProt Protein Name: Tubulin alpha-3C/D chain
• Protein Family: Tubulin
• UniProt Gene Name: TUBA3C
• UniProt Synonym Gene Names: TUBA2; TUBA3D
• UniProt Entry Name: TBA3C_HUMAN

NCBI Description

This gene encodes a member of the alpha tubulin family. Tubulin is a major component of microtubules, which are composed of alpha- and beta-tubulin heterodimers and microtubule-associated proteins in the cytoskeleton. Microtubules maintain cellular structure, function in intracellular transport, and play a role in spindle formation during mitosis. [provided by RefSeq, Oct 2011]

Uniprot Description

TUBA3C/D: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. Dimer of alpha and beta chains. Testis specific. Belongs to the tubulin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Cytoskeletal

Chromosomal Location of Human Ortholog: 13q11

Cellular Component: microtubule; cytoplasm; nucleus

Molecular Function: GTPase activity; protein binding; GTP binding; structural constituent of cytoskeleton

Biological Process: protein polymerization; 'de novo' posttranslational protein folding; cellular protein metabolic process; protein folding; microtubule-based process