Typical Testing Data/Standard Curve (for reference only)
Monkey Translocator protein ELISA Kit | TSPO elisa kit
Monkey Translocator protein ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: TSPO ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-TSPO antibody and an TSPO-HRP conjugate. The assay sample and buffer are incubated together with TSPO-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TSPO concentration since TSPO from samples and TSPO-HRP conjugate compete for the anti-TSPO antibody binding site. Since the number of sites is limited, as more sites are occupied by TSPO from the sample, fewer sites are left to bind TSPO-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TSPO concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
Present mainly in the mitochondrial compartment of peripheral tissues, the protein encoded by this gene interacts with some benzodiazepines and has different affinities than its endogenous counterpart. The protein is a key factor in the flow of cholesterol into mitochondria to permit the initiation of steroid hormone synthesis. Alternatively spliced transcript variants have been reported; one of the variants lacks an internal exon and is considered non-coding, and the other variants encode the same protein. [provided by RefSeq, Feb 2012]
Uniprot Description
TSPO: Responsible for the manifestation of peripheral-type benzodiazepine recognition sites and is most likely to comprise binding domains for benzodiazepines and isoquinoline carboxamides. May play a role in the transport of porphyrins and heme. Plays a role in the transport of cholesterol across mitochondrial membranes in steroidogenic cells. Belongs to the TspO/BZRP family. Interacts with BZRAP1. May interact with STAR. Interacts with MOST-1. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral; Membrane protein, multi-pass; Receptor, misc.; Mitochondrial; Apoptosis
Chromosomal Location of Human Ortholog: 22q13.31
Cellular Component: mitochondrial outer membrane; nuclear membrane; mitochondrion; intracellular membrane-bound organelle; cytoplasm; integral to membrane
Molecular Function: androgen binding; benzodiazepine receptor activity; cholesterol binding
Biological Process: steroid metabolic process; positive regulation of apoptosis; apoptosis; regulation of steroid biosynthetic process; axon regeneration in the peripheral nervous system; anion transport; response to vitamin B1; synaptic transmission; behavioral response to pain; positive regulation of mitochondrial depolarization; protein targeting to mitochondrion; aging; response to drug; adrenal gland development; negative regulation of nitric oxide biosynthetic process; chloride transport; negative regulation of tumor necrosis factor production; response to testosterone stimulus; lipid transport; glial cell migration; response to manganese ion; cell proliferation; response to progesterone stimulus; regulation of cholesterol transport; positive regulation of calcium ion transport; steroid biosynthetic process; heme biosynthetic process
Research Articles on TSPO
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Product Notes
The Monkey TSPO tspo (Catalog #AAA744476) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA744476 ELISA Kit recognizes Monkey TSPO. It is sometimes possible for the material contained within the vial of "Translocator protein, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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