SDS-Page
TRIP10 recombinant protein
TRIP10, 260-545aa, Human, His tag, E Coli
Gene Names
TRIP10; STP; CIP4; HSTP; STOT; TRIP-10
Applications
SDS-Page
Purity
> 90% by SDS-PAGE
Synonyms
TRIP10; 260-545aa; Human; His tag; E Coli; Cdc42-interacting protein 4; CIP4; HSTP; STOT; STP; TRIP10 recombinant protein
Host
E Coli
Purity/Purification
> 90% by SDS-PAGE
Form/Format
Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol, 1mM DTT
Concentration
1 mg/ml (determined by Bradford assay) (varies by lot)
Sequence
MGSSHHHHHH SSGLVPRGSH MGSDPKNDSH VLIELHKSGF ARPGDVEFED FSQPMNRAPS DSSLGTPSDG RPELRGPGRS RTKRWPFGKK NKTVVTEDFS HLPPEQQRKR LQQQLEERSR ELQKEVDQRE ALKKMKDVYE KTPQMGDPAS LEPQIAETLS NIERLKLEVQ KYEAWLAEAE SRVLSNRGDS LSRHARPPDP PASAPPDSSS NSASQDTKES SEEPPSEESQ DTPIYTEFDE DFEEEPTSPI GHCVAIYHFE GSSEGTISMA EGEDLSLMEE DKGDGWTRVR RKEGGEGYVP TSYLRVTLN
Sequence Length
545
Applicable Applications for TRIP10 recombinant protein
SDS-PAGE
Antigen Species
Human
Tag Information
His-tag
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
SDS-Page
SDS-Page
Related Product Information for TRIP10 recombinant protein
Cdc42-interacting protein 4, also known as TRIP10, belongs to the F-BAR family of proteins. These proteins contain an N-terminal, alpha-helical, region that is hydrophobic and considered to be related to the Bin Amphiphysin Rvs (BAR) protein family. Expressed in a variety of tissues, including kidney, brain, liver, lung, heart and pancreas, TRIP10 is required for the Insulin-dependent translocation of Glut4 to the plasma membrane and is essential for the coordination of membrane tubulation with Actin cytoskeletal reorganization during endocytosis. Recombinant human TRIP10 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.
Product Categories/Family for TRIP10 recombinant protein
References
Aspenstrom P., et al. (1997) Curr Biol. 7: 479-487.; Holbert S., et al. (2003) Proc Natl Acad Sci uSA. 100: 2712-2717.;
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
34.6kDa (309aa), confirmed by MALDI-TOF (Molecular size on SDS-PAGE will appear higher)
NCBI Official Full Name
cdc42-interacting protein 4 isoform 2
NCBI Official Synonym Full Names
thyroid hormone receptor interactor 10
NCBI Official Symbol
TRIP10
NCBI Official Synonym Symbols
STP; CIP4; HSTP; STOT; TRIP-10
NCBI Protein Information
cdc42-interacting protein 4
UniProt Protein Name
Cdc42-interacting protein 4
Protein Family
UniProt Gene Name
TRIP10
UniProt Synonym Gene Names
CIP4; STOT; STP; hSTP; TR-interacting protein 10; TRIP-10
Uniprot Description
Required for translocation of GLUT4 to the plasma membrane in response to insulin signaling (). Required to coordinate membrane tubulation with reorganization of the actin cytoskeleton during endocytosis. Binds to lipids such as phosphatidylinositol 4,5-bisphosphate and phosphatidylserine and promotes membrane invagination and the formation of tubules. Also promotes CDC42-induced actin polymerization by recruiting WASL/N-WASP which in turn activates the Arp2/3 complex. Actin polymerization may promote the fission of membrane tubules to form endocytic vesicles. Required for the formation of podosomes, actin-rich adhesion structures specific to monocyte-derived cells. May be required for the lysosomal retention of FASLG/FASL.
Research Articles on TRIP10
Similar Products
Product Notes
The TRIP10 trip10 (Catalog #AAA205136) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The tag for this protein is His-tag. AAA Biotech's TRIP10 can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the TRIP10 trip10 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MGSSHHHHHH SSGLVPRGSH MGSDPKNDSH VLIELHKSGF ARPGDVEFED FSQPMNRAPS DSSLGTPSDG RPELRGPGRS RTKRWPFGKK NKTVVTEDFS HLPPEQQRKR LQQQLEERSR ELQKEVDQRE ALKKMKDVYE KTPQMGDPAS LEPQIAETLS NIERLKLEVQ KYEAWLAEAE SRVLSNRGDS LSRHARPPDP PASAPPDSSS NSASQDTKES SEEPPSEESQ DTPIYTEFDE DFEEEPTSPI GHCVAIYHFE GSSEGTISMA EGEDLSLMEE DKGDGWTRVR RKEGGEGYVP TSYLRVTLN. It is sometimes possible for the material contained within the vial of "TRIP10, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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