Human tripartite motif-containing 63 ELISA Kit | TRIM63 elisa kit

Human E3 ubiquitin-protein ligase TRIM63, TRIM63 ELISA Kit

Cat. Num. AAA947128

• Gene Names: TRIM63; IRF; SMRZ; MURF1; MURF2; RNF28
• Reactivity: Human
• Synonyms: tripartite motif-containing 63; Human E3 ubiquitin-protein ligase TRIM63; TRIM63 ELISA Kit; Human E3 ubiquitin-protein ligase TRIM63 (TRIM63) ELISA kit; FLJ32380; IRF; MURF1; MURF2; RNF28; SMRZ; iris ring finger protein; muscle specific ring finger protein 1; muscle specific ring finger protein 2; ring finger protein 28; striated muscle RING zinc finger; TRIM63 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human TRIM63. No significant cross-reactivity or interference between human TRIM63 and analogues was observed.
• Sequence Length: 353

• Samples: Serum, plasma, tissue homogenates, cell lysates
• Assay Type: Quantitative Sandwich
• Detection Range: 12.5 pg/ml-800 pg/ml
• Sensitivity: < 3.1 pg/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TRIM63 elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TRIM63 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TRIM63 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TRIM63 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TRIM63 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 19924163
• NCBI GeneID: 84676
• NCBI Accession #: NP_115977.2
• NCBI GenBank Nucleotide #: NM_032588.3
• UniProt Accession #: Q969Q1; Q5T2I1; Q96BD3; Q96KD9; Q9BYV4; B4DN95
• Molecular Weight: 40,248 Da
• NCBI Official Full Name: E3 ubiquitin-protein ligase TRIM63
• NCBI Official Synonym Full Names: tripartite motif containing 63, E3 ubiquitin protein ligase
• NCBI Official Symbol: TRIM63
• NCBI Official Synonym Symbols: IRF; SMRZ; MURF1; MURF2; RNF28
• NCBI Protein Information: E3 ubiquitin-protein ligase TRIM63; ring finger protein 28; iris ring finger protein; muscle specific ring finger protein 2; muscle-specific RING finger protein 1; tripartite motif-containing protein 63; striated muscle RING zinc finger protein
• UniProt Protein Name: E3 ubiquitin-protein ligase TRIM63
• Protein Family: E3 ubiquitin-protein ligase
• UniProt Gene Name: TRIM63
• UniProt Synonym Gene Names: IRF; MURF1; RNF28; SMRZ; MuRF-1; MuRF1
• UniProt Entry Name: TRI63_HUMAN

NCBI Description

This gene encodes a member of the RING zinc finger protein family found in striated muscle and iris. The product of this gene is an E3 ubiquitin ligase that localizes to the Z-line and M-line lattices of myofibrils. This protein plays an important role in the atrophy of skeletal and cardiac muscle and is required for the degradation of myosin heavy chain proteins, myosin light chain, myosin binding protein, and for muscle-type creatine kinase. [provided by RefSeq, Feb 2012]

Uniprot Description

MURF1: E3 ubiquitin ligase. Regulates proteasomal degradation of cardiac troponin I/TNNI3 and probably of other sarcomeric- associated proteins. May play a role in striated muscle atrophy and hypertrophy by regulating an anti-hypertrophic PKC-mediated signaling pathway. May regulate the organization of myofibrils through TTN in muscle cells.

Protein type: Ligase; Ubiquitin conjugating system; EC 6.3.2.-; EC 6.3.2.19; Ubiquitin ligase

Chromosomal Location of Human Ortholog: 1p34-p33

Cellular Component: microtubule; cytoplasm; M band; nucleus; Z disc

Molecular Function: protein binding; signal transducer activity; zinc ion binding; ubiquitin-protein ligase activity; titin binding; ligase activity

Biological Process: proteasomal ubiquitin-dependent protein catabolic process; skeletal muscle atrophy; muscle contraction; regulation of gene expression; protein ubiquitination; response to electrical stimulus involved in regulation of muscle adaptation; signal transduction