Human TRIM14FALSE ELISA Kit | TRIM14 elisa kit

Human TRIM14FALSE ELISA Kit

Cat. Num. AAA7607001

• Reactivity: Human
• Synonyms: TRIM14FALSE; Human TRIM14FALSE ELISA Kit; TRIM14 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of TRIM14. No significant cross-reactivity or interference between TRIM14 and analogues was observed.
• Sequence Length: 442

• Samples: serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.156-10ng/ml
• Sensitivity: <0.094ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level TRIM14 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level TRIM14 were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
• Preparation and Storage: Store at 4 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TRIM14 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- TRIM14 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- TRIM14 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the TRIM14 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of TRIM14 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 15208665
• NCBI GeneID: 9830
• NCBI Accession #: NP_150088.1
• NCBI GenBank Nucleotide #: NM_033219.2
• UniProt Accession #: Q14142; Q548W9; Q5TBQ8; Q6ZWL7; Q9BRD8; Q9C020; A8K9W0; E7EQC4; F8W956
• Molecular Weight: 23,146 Da
• NCBI Official Full Name: tripartite motif-containing protein 14
• NCBI Official Synonym Full Names: tripartite motif containing 14
• NCBI Official Symbol: TRIM14
• NCBI Protein Information: tripartite motif-containing protein 14
• UniProt Protein Name: Tripartite motif-containing protein 14
• Protein Family: Tripartite motif-containing protein
• UniProt Gene Name: TRIM14
• UniProt Synonym Gene Names: KIAA0129

NCBI Description

The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to cytoplasmic bodies and its function has not been determined. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2010]

Uniprot Description

Plays a role in the innate immune defense against viruses. Facilitates the type I IFN response by interacting with MAVS at the outer mitochondria membrane and thereby recruiting NF-kappa-B essential modulator IKBKG/NEMO to the MAVS signalosome, leading to the activation of both the IFN regulatory factor 3/IRF3 and NF-kappa-B pathways (PubMed:24379373). Positively regulates the CGAS-induced type I interferon signaling pathway by stabilizing CGAS and inhibiting its autophagic degradation (PubMed:27666593).