TREM-2 Recombinant Protein | TREM2 recombinant protein

TREM-2 Protein, Mouse, Recombinant (hFc Tag)

Cat. Num. AAA8122563

• Gene Names: Trem2; TREM-2; Trem2a; Trem2b; Trem2c
• Purity: >90% as determined by SDS-PAGE
• Synonyms: TREM-2; TREM-2 Protein; Mouse; Recombinant (hFc Tag); Mouse TREM-2/TREM2 Protein (Fc Tag); Trem2a Protein; Trem2b Protein; Trem2c Protein; triggering receptor expressed on myeloid cells 2; TREM2 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Pro168

• Species: Mouse
• Endotoxin: <1.0EU per ug protein as determined by the LAL method.
• Predicted N Terminal: Leu 19
• Tag: C-human IgG1-Fc
• Protein Construction: A DNA sequence encoding the mouse Trem2 (NP_112544.1)(Met1-Pro168) was expressed with the Fc region of human IgG1 at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for TREM2 recombinant protein

Background: Triggering receptor expressed on myeloid cells 2 (TREM2) is a single Ig domain receptor. It is expressed on macrophages and dendritic cells but not on granulocytes or monocytes. Its expression is most abundant in the basal ganglia, corpus callosum, medulla oblongata and spinal cord, and microglial cells are the major TREM2-producing cell type in the central nervous system (CNS). TREM2 may play a role in chronic inflammations and may stimulate production of constitutive rather than inflammatory chemokines and cytokines. TREM2 forms a receptor signaling complex with TYROBP and triggers activation of the immune responses in macrophages and dendritic cells. It also associates with the signal adapter protein, DAP12, which has a cytoplasmic ITAM, leading to the subsequent activation of cytoplasmic tyrosine kinases. TREM2 is both required and sufficient for competent uptake of apoptotic neuronal cells. TREM2 and TREM2-L form a receptor-ligand pair connecting microglia with apoptotic neurons, directing removal of damaged cells to allow repair. Deficiency of the adapter protein DAP12 or its associated receptor TREM2 is associated with abnormal osteoclast development in humans. Defects in TREM2 are causes of PLOSL, also known as NHD. In addition, TREM2 signaling is also an important pathway to promote healing of wounds in the colon where stem cell replacement is necessary.

References

Bouchon, A. et al., 2000, J. Immunol. 164: 4991-4995. Paloneva, J. et al., 2002, Am. J. Hum. Genet. 71:656-662. Prada, I. et al., 2006, Neuroscience. 140 (4): 1139-48. Neumann, H. et al., 2007, J Neuroimmunol. 184 (1-2): 92-9. Thrash, JC. et al., 2009, Neurochem Res. 34 (1): 38-45.

NCBI and Uniprot Product Information

• NCBI GI #: 13994125
• NCBI GeneID: 83433
• NCBI Accession #: NP_112544.1
• NCBI GenBank Nucleotide #: NM_031254.3
• NCBI Official Full Name: triggering receptor expressed on myeloid cells 2 isoform 1
• NCBI Official Synonym Full Names: triggering receptor expressed on myeloid cells 2
• NCBI Official Symbol: Trem2
• NCBI Official Synonym Symbols: TREM-2; Trem2a; Trem2b; Trem2c
• NCBI Protein Information: triggering receptor expressed on myeloid cells 2
• Protein Family: Triggering receptor expressed on myeloid cells

NCBI Description

The protein encoded by this gene is part of the immunoglobulin and lectin-like superfamily and functions as part of the innate immune system. This gene forms part of a cluster of genes on mouse chromosome 17 thought to be involved in innate immunity. This protein associates with the adaptor protein Dap-12 and recruits several factors, such as kinases and phospholipase C-gamma, to form a receptor signaling complex that activates myeloid cells, including dendritic cells and microglia. In humans homozygous loss-of-function mutations in this gene cause Nasu-Hakola disease and mutations in this gene may be risk factors to the development of Alzheimer's disease. In mouse mutations of this gene serve as a pathophysiological model for polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy (Nasu-Hakola disease) and for inflammatory bowel disease. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, Jan 2013]