TNF Receptor Associated Factor 4 Recombinant Protein | TRAF4 recombinant protein

Recombinant TNF Receptor Associated Factor 4 (TRAF4)

Cat. Num. AAA2030811

• Gene Names: TRAF4; CART1; MLN62; RNF83
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >95%
• Synonyms: TNF Receptor Associated Factor 4; Recombinant TNF Receptor Associated Factor 4 (TRAF4); TRAF4 recombinant protein

• Host: Host: E Coli; Source: Prokaryotic expression
• Purity/Purification: >95%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Concentration: 8.5 (varies by lot)
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- QPCTYCTK EFVFDTIQSH QYQCPRLPVA CPNQCGVGTV AREDLPGHLK DSCNTALVLC PFKDSGCKHR CPKLAMARHV EESVKPHLAM MCALVSRQRQ ELQELRRELE ELSVGSDGVL IWKIGSYGRR LQEAKAKPNL ECFSPAFYTH KYGYKLQVSA FLNGNGSGEG THLSLYIRVL PGAFDNLLEW PFARRVTFSLLDQSDPGLAK PQHVTETFHP DPNWKNFQKP GTWRGSLDES SLGFGYPKFI SHQD
• Sequence Length: 470

• Applicable Applications for TRAF4 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Predicted Molecular Mass: 32.2kDa
• Accurate Molecular Mass (KD): 30kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Tag: two N-terminal Tags, His-tag and T7-tag
• Organism Species: Homo sapiens (Human)
• Fragment: Gln193~Asp444
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

Sequence Information

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 22027622
• NCBI GeneID: 9618
• NCBI Accession #: NP_004286.2
• NCBI GenBank Nucleotide #: NM_004295.3
• Molecular Weight: 22,841 Da
• NCBI Official Full Name: TNF receptor-associated factor 4
• NCBI Official Synonym Full Names: TNF receptor-associated factor 4
• NCBI Official Symbol: TRAF4
• NCBI Official Synonym Symbols: CART1; MLN62; RNF83
• NCBI Protein Information: TNF receptor-associated factor 4
• UniProt Protein Name: TNF receptor-associated factor 4
• Protein Family: TNF receptor-associated factor
• UniProt Gene Name: TRAF4
• UniProt Synonym Gene Names: CART1; MLN62; RNF83; MLN 62
• UniProt Entry Name: TRAF4_HUMAN

NCBI Description

This gene encodes a member of the TNF receptor associated factor (TRAF) family. TRAF proteins are associated with, and mediate signal transduction from members of the TNF receptor superfamily. The encoded protein has been shown to interact with neurotrophin receptor, p75 (NTR/NTSR1), and negatively regulate NTR induced cell death and NF-kappa B activation. This protein has been found to bind to p47phox, a cytosolic regulatory factor included in a multi-protein complex known as NAD(P)H oxidase. This protein thus, is thought to be involved in the oxidative activation of MAPK8/JNK. Alternatively spliced transcript variants have been observed but the full-length nature of only one has been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

TRAF4: Adapter protein and signal transducer that links members of the tumor necrosis factor receptor (TNFR) family to different signaling pathways. Plays a role in the activation of NF-kappa-B and JNK, and in the regulation of cell survival and apoptosis. Regulates activation of NF-kappa-B in response to signaling through Toll-like receptors. Required for normal skeleton development, and for normal development of the respiratory tract. Required for activation of RPS6KB1 in response to TNF signaling. Modulates TRAF6 functions. Belongs to the TNF receptor-associated factor family. B subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Adaptor/scaffold; DNA-binding

Chromosomal Location of Human Ortholog: 17q11-q12

Cellular Component: tight junction; cytoskeleton; perinuclear region of cytoplasm; cytoplasm; plasma membrane; nucleus

Molecular Function: protein binding; DNA binding; zinc ion binding; ubiquitin protein ligase binding; thioesterase binding; WW domain binding; ubiquitin-protein ligase activity; tumor necrosis factor receptor binding; protein kinase binding

Biological Process: regulation of apoptosis; apoptosis; positive regulation of protein kinase activity; respiratory tube development; activation of NF-kappaB-inducing kinase; positive regulation of JNK cascade; protein ubiquitination; respiratory gaseous exchange; signal transduction