Mouse TNF receptor-associated factor 2 ELISA Kit | TRAF2 elisa kit

Mouse TNF receptor-associated factor 2, TRAF2 ELISA Kit

Cat. Num. AAA9331805

• Gene Names: Traf2; AI325259
• Reactivity: Mouse
• Synonyms: TNF receptor-associated factor 2; Mouse TNF receptor-associated factor 2; TRAF2 ELISA Kit; Mouse TNF receptor-associated factor 2 (TRAF2) ELISA kit; MGC:45012; TRAP; TRAP3; tumor necrosis factor type 2 receptor associated protein 3; TRAF2 elisa kit

• Reactivity: Mouse

• Samples: Undiluted original Mouse serum, plasma or tissue homogenates samples
• Assay Type: Quantitative Sandwich
• Detection Range: 62.5pg/ml-2000pg/ml
• Sensitivity: 10pg/ml
• Intra-assay Precision: Intra-assay CV (%) is less than 15%.
• Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]. All CV% should be compared by concentration, not compared by OD values.
• Preparation and Storage: Store all reagents at 2-8 degree C

Related Product Information for TRAF2 elisa kit

Background/Introduction: This Quantitative Sandwich ELISA kit is for lab reagent/research use only! This kit is intended to be used for determine the level of TRAF2 (hereafter termed "analyte") in undiluted original Mouse serum, plasma or tissue homogenates samples. For other sample types please contact tech support to determine compatibility with this assay. This kit is not suitable for assaying non-biological sources of substances.

NCBI and Uniprot Product Information

• NCBI GI #: 594150400
• NCBI GeneID: 22030
• NCBI Accession #: NP_001277342.1
• NCBI GenBank Nucleotide #: NM_001290413.1
• UniProt Accession #: P39429; O54896; A2AJA3
• Molecular Weight: 56,026 Da
• NCBI Official Full Name: TNF receptor-associated factor 2 isoform a
• NCBI Official Synonym Full Names: TNF receptor-associated factor 2
• NCBI Official Symbol: Traf2
• NCBI Official Synonym Symbols: AI325259
• NCBI Protein Information: TNF receptor-associated factor 2; E3 ubiquitin-protein ligase TRAF2
• UniProt Protein Name: TNF receptor-associated factor 2
• Protein Family: TNF receptor-associated factor
• UniProt Gene Name: Traf2
• UniProt Entry Name: TRAF2_MOUSE

Uniprot Description

TRAF2: Regulates activation of NF-kappa-B and JNK and plays a central role in the regulation of cell survival and apoptosis. Required for normal antibody isotype switching from IgM to IgG. Has E3 ubiquitin-protein ligase activity and promotes 'Lys-63'- linked ubiquitination of target proteins, such as BIRC3, RIPK1 and TICAM1. Is an essential constituent of several E3 ubiquitin- protein ligase complexes, where it promotes the ubiquitination of target proteins by bringing them into contact with other E3 ubiquitin ligases. Regulates BIRC2 and BIRC3 protein levels by inhibiting their autoubiquitination and subsequent degradation; this does not depend on the TRAF2 RING-type zinc finger domain. Homotrimer, and heterotrimer with TRAF1 and TRAF3 (via TRAF domain). The domain containing the RING-type and the first TRAF-type zinc finger can also form homodimers (in vitro). Interacts with TNFRSF1B/TNFR2, TNFRSF4, TNFRSF5/CD40, CD27/TNFRSF7, TNFRSF8/CD30, TNFRSF9/CD137, TNFRSF11A/RANK, TNFRSF13B/TACI, TNFRSF14, TNFRSF16/NGFR, TNFRSF17/BCMA, TNFRSF18/AITR, TNFRSF19/TROY, TNFRSF19L/RELT, XEDAR, EDAR, Epstein-Barr virus BNFL1/LMP-1 and IL15RA. Interacts with CDK9, CSK, MAP3K1, MAP3K5, MAP3K11, MAP3K14, MAP4K2, RIPK1, RIPK2, TNIK, TBK1, SPHK1, TRADD, TRAFD1, TRAIP, TANK/ITRAF, TNFAIP3, TDP2, MAVS/IPS1, TICAM1 and TRPC4AP. Interacts with CASP8AP2, NFATC2IP, PEG3 and HIVEP3. Interacts with BIRC2 and BIRC3 N- terminus; a single BIRC2 or BIRC3 molecule interacts with a heterotrimer formed by TRAF1 and TRAF2, or a TRAF2 homotrimer. Identified in a complex composed of TRAF2, TRAF3, BIRC2 and BIRC3. Interaction with BIRC2 and/or BIRC3 is essential for degradation of NFKBIA and activation of NF-kappa-B. Interacts with CYLD, USP48, DAB2IP, IKKA and IKKB. Identified in a complex with TNFRSF1A, RIPK1 and IKKB. Interacts (via 'Lys-63'-linked polyubiquitin chains) with TAB2 and TAB3. Interacts with ERN1 and TAOK3. Interaction with TAOK3 is facilitated under ER stress conditions, such as treatment with tunicamycin, and may promote TRAF2 phosphorylation. Has very low E3 ubiquitin ligase activity in the absence of sphingosine-1-phosphate. E3 ubiquitin ligase activity is strongly activated by cytoplasmic sphingosine-1- phosphate. Belongs to the TNF receptor-associated factor family. A subfamily. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin ligase; EC 6.3.2.-; Ligase; Ubiquitin conjugating system; Autophagy

Cellular Component: internal side of plasma membrane; protein complex; cytoplasm; cell cortex; vesicle membrane; cytosol; ubiquitin ligase complex; lipid raft

Molecular Function: sphingolipid binding; identical protein binding; zinc ion binding; coenzyme F420-2 alpha-glutamyl ligase activity; metal ion binding; ubiquitin-protein ligase activity; ribosomal S6-glutamic acid ligase activity; CD40 receptor binding; mitogen-activated protein kinase kinase kinase binding; tumor necrosis factor receptor binding; protein phosphatase binding; protein binding; enzyme binding; UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity; thioesterase binding; ubiquitin protein ligase binding; protein complex binding; coenzyme F420-0 gamma-glutamyl ligase activity; lipid binding; ligase activity; receptor binding

Biological Process: positive regulation of JNK activity; protein autoubiquitination; protein heterooligomerization; apoptosis; activation of NF-kappaB-inducing kinase; positive regulation of T cell cytokine production; signal transduction; regulation of JNK cascade; activation of NF-kappaB transcription factor; regulation of apoptosis; cellular protein complex assembly; tumor necrosis factor-mediated signaling pathway; positive regulation of interleukin-2 production; protein complex assembly; protein catabolic process; positive regulation of transcription factor activity; regulation of immunoglobulin secretion; negative regulation of apoptosis