Human TNF receptor-associated factor 1 ELISA Kit | TRAF1 elisa kit

Human tumor necrosis factor-associated factor 1, TRAF1 ELISA Kit

Cat. Num. AAA701814

• Gene Names: TRAF1; EBI6; MGC:10353
• Reactivity: Human
• Synonyms: TNF receptor-associated factor 1; Human tumor necrosis factor-associated factor 1; TRAF1 ELISA Kit; TRAF1 ELISA kit; EBI6; MGC:10353; Epstein-Bar virus-induced protein 6; TRAF1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human TRACP-5b. No significant cross-reactivity or interference between human TRACP-5b and analogues was observed.
• Sequence Length: 416

• Samples: Serum, Plasma, Tissue Homogenates
• Assay Type: Quantitative Sandwich
• Detection Range: 0.312 mIU/ml-20 mIU/ml
• Sensitivity: 0.078 mIU/ml.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TRAF1 elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TRACP-5b has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TRACP-5b present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TRACP-5b is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TRACP-5b bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 300193045
• NCBI GeneID: 7185
• NCBI Accession #: NP_001177874.1
• NCBI GenBank Nucleotide #: NM_001190945.1
• UniProt Accession #: Q13077; Q658U1; Q8NF13; B4DJ77
• Molecular Weight: 46,164 Da
• NCBI Official Full Name: TNF receptor-associated factor 1 isoform a
• NCBI Official Synonym Full Names: TNF receptor-associated factor 1
• NCBI Official Symbol: TRAF1
• NCBI Official Synonym Symbols: EBI6; MGC:10353
• NCBI Protein Information: TNF receptor-associated factor 1; Epstein-Bar virus-induced protein 6
• UniProt Protein Name: TNF receptor-associated factor 1
• Protein Family: TNF receptor-associated factor
• UniProt Gene Name: TRAF1
• UniProt Synonym Gene Names: EBI6
• UniProt Entry Name: TRAF1_HUMAN

NCBI Description

The protein encoded by this gene is a member of the TNF receptor (TNFR) associated factor (TRAF) protein family. TRAF proteins associate with, and mediate the signal transduction from various receptors of the TNFR superfamily. This protein and TRAF2 form a heterodimeric complex, which is required for TNF-alpha-mediated activation of MAPK8/JNK and NF-kappaB. The protein complex formed by this protein and TRAF2 also interacts with inhibitor-of-apoptosis proteins (IAPs), and thus mediates the anti-apoptotic signals from TNF receptors. The expression of this protein can be induced by Epstein-Barr virus (EBV). EBV infection membrane protein 1 (LMP1) is found to interact with this and other TRAF proteins; this interaction is thought to link LMP1-mediated B lymphocyte transformation to the signal transduction from TNFR family receptors. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jul 2010]

Uniprot Description

Function: Adapter molecule that regulates the activation of NF-kappa-B and JNK. Plays a role in the regulation of cell survival and apoptosis. The heterotrimer formed by TRAF1 and TRAF2 is part of a E3 ubiquitin-protein ligase complex that promotes ubiquitination of target proteins, such as MAP3K14. The TRAF1/TRAF2 complex recruits the antiapoptotic E3 protein-ubiquitin ligases BIRC2 and BIRC3 to TNFRSF1B/TNFR2.6 PublicationsManual assertion based on experiment in:Ref.18