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Coupling protein TraD (traD) Recombinant Protein | traD recombinant protein

Recombinant Escherichia coli Coupling protein TraD (traD)

Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
Synonyms
Coupling protein TraD (traD); Recombinant Escherichia coli Coupling protein TraD (traD); Recombinant Coupling protein TraD (traD); Coupling protein TraD; DNA transport protein TraD; traD recombinant protein
Ordering
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Purity/Purification
Greater or equal to 85% purity as determined by SDS-PAGE.
Form/Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Sequence Positions
1-717
Sequence
MSFNAKDMTQGGQIASMRIRMFSQIANIMLYCLFIFFWILVGLVLWIKISWQTFVNGCIYWWCTTLEGMRDLIKSQPVYEIQYYGKTFRMNAAQVLHDKYMIWCSEQLWSAFVLAAVVALVICLITFFVVSWILGRQGKQQSENEVTGGRQLTDNPKDVARMLKKDGKDSDIRIGDLPIIRDSEIQNFCLHGTVGAGKSEVIRRLANYARQRGDMVVIYDRSGEFVKSYYDPSIDKILNPLDARCAAWDLWKECLTQPDFDNTANTLIPMGTKEDPFWQGSGRTIFAEAAYLMRNDPNRSYSKLVDTLLSIKIEKLRTYLRNSPAANLVEEKIEKTAISIRAVLTNYVKAIRYLQGIEHNGEPFTIRDWMRGVREDQKNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRVWFFCDELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGEKAAASLFDVMNTRAFFRSPSHKIAEFAAGEIGEKEHLKASEQYSYGADPVRDGVSTGKDMERQTLVSYSDIQSLPDLTCYVTLPGPYPAVKLSLKYQTRPKVAPEFIPRDINPEMENRLSAVLAAREAEGRQMASLFEPDVPEVVSGEDVTQAEQPQQPVSPAINDKKSDSGVNVPAGGIEQELKMKPEEEMEQQLPPGISESGEVVDMAAYEAWQQENHPDIQQQMQRREEVNINVHRERGEDVEPGDDF
Sequence Length
717
Species
Escherichia coli (strain K12)
Preparation and Storage
Store at -20 degree C. For extended storage, store at -20 or -80 degree C.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
81,489 Da
NCBI Official Full Name
conjugal transfer protein TraD
NCBI Official Symbol
traD
NCBI Protein Information
conjugal transfer protein TraD
UniProt Protein Name
Coupling protein TraD
Protein Family
UniProt Gene Name
traD
UniProt Entry Name
TRAD1_ECOLI

Uniprot Description

Function: Conjugative DNA transfer (CDT) is the unidirectional transfer of ssDNA plasmid from a donor to a recipient cell. It is the central mechanism by which antibiotic resistance and virulence factors are propagated in bacterial populations. Couples the transferosome to a type IV secretion system. Probably forms a pore through which single-stranded plasmid DNA is transferred to the secretion system. The last 37 residues are important for determining plasmid specificity and transfer efficiency, with additional specificity conferred by the TraD-TraM pair. Ref.12

Subunit structure: Interacts with relaxosome component TraM. May form a hexamer in the membrane. Ref.11 Ref.14

Subcellular location: Cell inner membrane; Multi-pass membrane protein Ref.11.

Sequence similarities: Belongs to the TrwB coupling protein family.

Sequence caution: The sequence AAC44181.2 differs from that shown. Reason: Erroneous termination at position 718. Translated as stop.The sequence CAA30013.1 differs from that shown. Reason: Frameshift at position 19.

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Product Notes

The traD trad (Catalog #AAA1080500) is a Recombinant Protein produced from E Coli or Yeast or Baculovirus or Mammalian Cell and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 1-717. The amino acid sequence is listed below: MSFNAKDMTQ GGQIASMRIR MFSQIANIML YCLFIFFWIL VGLVLWIKIS WQTFVNGCIY WWCTTLEGMR DLIKSQPVYE IQYYGKTFRM NAAQVLHDKY MIWCSEQLWS AFVLAAVVAL VICLITFFVV SWILGRQGKQ QSENEVTGGR QLTDNPKDVA RMLKKDGKDS DIRIGDLPII RDSEIQNFCL HGTVGAGKSE VIRRLANYAR QRGDMVVIYD RSGEFVKSYY DPSIDKILNP LDARCAAWDL WKECLTQPDF DNTANTLIPM GTKEDPFWQG SGRTIFAEAA YLMRNDPNRS YSKLVDTLLS IKIEKLRTYL RNSPAANLVE EKIEKTAISI RAVLTNYVKA IRYLQGIEHN GEPFTIRDWM RGVREDQKNG WLFISSNADT HASLKPVISM WLSIAIRGLL AMGENRNRRV WFFCDELPTL HKLPDLVEIL PEARKFGGCY VFGIQSYAQL EDIYGEKAAA SLFDVMNTRA FFRSPSHKIA EFAAGEIGEK EHLKASEQYS YGADPVRDGV STGKDMERQT LVSYSDIQSL PDLTCYVTLP GPYPAVKLSL KYQTRPKVAP EFIPRDINPE MENRLSAVLA AREAEGRQMA SLFEPDVPEV VSGEDVTQAE QPQQPVSPAI NDKKSDSGVN VPAGGIEQEL KMKPEEEMEQ QLPPGISESG EVVDMAAYEA WQQENHPDIQ QQMQRREEVN INVHRERGED VEPGDDF. It is sometimes possible for the material contained within the vial of "Coupling protein TraD (traD), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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