SDS-PAGE
TPX2 recombinant protein
TPX2 recombinant protein
Gene Names
TPX2; DIL2; p100; DIL-2; HCTP4; FLS353; HCA519; REPP86; C20orf1; C20orf2; GD:C20orf1
Applications
Western Blot
Purity
>80%
Synonyms
TPX2; TPX2 recombinant protein; DIL2; p100; DIL-2; HCTP4; FLS353; HCA519; REPP86; C20orf1; C20orf2; GD:C20orf1
Host
Sf9 insect cells
Purity/Purification
>80%
Form/Format
50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, 25% glycerol.
Concentration
0.2ug/ul (varies by lot)
Sequence Length
3685
Applicable Applications for TPX2 recombinant protein
Kinase Assay, Western Blot (WB)
Type
Recombinant Fusion Protein
Species
Human
Tag Information
His tag
Expression System
Sf9 insect cells using baculovirus
Source Note
Recombinant full-length human TPX2 protein was expressed by baculovirus in Sf9 insect cells. This product can be used for Aurora A activation in vitro.
Preparation and Storage
Store product at -70 degree C. For optimal storage, aliquot target into smaller quantitites after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles. Product shipped on Dry Ice.
SDS-PAGE
SDS-PAGE
Related Product Information for TPX2 recombinant protein
Recombinant full-length human TPX2 protein was expressed by baculovirus in Sf9 insect cells using an N-terminal His tag. This product can be used for Aurora A activation in vitro.
Scientific Background: TPX2 is a nuclear proliferation-associated protein whose expression is restricted to cell cycle phases S, G2, and M (1). It mainly expressed in the lung carcinoma cell lines and also found in adult placenta, skeletal muscle, thymus, testis, and small intestine and in fetal brain, liver, and kidney.TPX2 is required for targeting STK6 to the spindle apparatus, and that STK6 may regulate the function of TPX2 during spindle assembly. TPX2 is mailnly function in mitotic spindle assembly likely contributes to its role in chromosome stability control and tumor suppression (2).
Scientific Background: TPX2 is a nuclear proliferation-associated protein whose expression is restricted to cell cycle phases S, G2, and M (1). It mainly expressed in the lung carcinoma cell lines and also found in adult placenta, skeletal muscle, thymus, testis, and small intestine and in fetal brain, liver, and kidney.TPX2 is required for targeting STK6 to the spindle apparatus, and that STK6 may regulate the function of TPX2 during spindle assembly. TPX2 is mailnly function in mitotic spindle assembly likely contributes to its role in chromosome stability control and tumor suppression (2).
Product Categories/Family for TPX2 recombinant protein
References
1. Heidebrecht, H. J. et.al: p100: a novel proliferation-associated nuclear protein specifically restricted to cell cycle phases S, G2, and M. Blood 90: 226-233, 1997.
2. Joukov, V.et.al: The BRCA1/BARD1 heterodimer modulates Ran-dependent mitotic spindle assembly. Cell 127: 539-552, 2006.
2. Joukov, V.et.al: The BRCA1/BARD1 heterodimer modulates Ran-dependent mitotic spindle assembly. Cell 127: 539-552, 2006.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
~110 kDa
NCBI Official Full Name
Homo sapiens TPX2, microtubule-associated (TPX2), mRNA
NCBI Official Synonym Full Names
TPX2, microtubule-associated
NCBI Official Symbol
TPX2
NCBI Official Synonym Symbols
DIL2; p100; DIL-2; HCTP4; FLS353; HCA519; REPP86; C20orf1; C20orf2; GD:C20orf1
NCBI Protein Information
targeting protein for Xklp2; protein fls353; TPX2, microtubule-associated, homolog; differentially expressed in lung cells; TPX2, microtubule-associated protein homolog; preferentially expressed in colorectal cancer; hepatocellular carcinoma-associated antigen 519; restricted expression proliferation associated protein 100; restricted expression proliferation-associated protein 100; differentially expressed in cancerous and non-cancerous lung cells 2
UniProt Protein Name
Targeting protein for Xklp2
Protein Family
UniProt Gene Name
TPX2
UniProt Synonym Gene Names
C20orf1; C20orf2; DIL2; HCA519; DIL-2; p100
UniProt Entry Name
TPX2_HUMAN
Uniprot Description
Function: Spindle assembly factor. Required for normal assembly of mitotic spindles. Required for normal assembly of microtubules during apoptosis. Required for chromatin and/or kinetochore dependent microtubule nucleation. Mediates AURKA localization to spindle microtubules. Activates AURKA by promoting its autophosphorylation at 'Thr-288' and protects this residue against dephosphorylation. Ref.13 Ref.17
Subunit structure: Interacts with AURKA. Ref.13 Ref.22 Ref.23
Subcellular location: Nucleus. Cytoplasm › cytoskeleton › spindle. Cytoplasm › cytoskeleton › spindle pole. Note: During mitosis it is strictly associated with the spindle pole and with the mitotic spindle, whereas during S and G2, it is diffusely distributed throughout the nucleus. Is released from the nucleus in apoptotic cells and is detected on apoptotic microtubules. Ref.13 Ref.17
Tissue specificity: Expressed in lung carcinoma cell lines but not in normal lung tissues.
Developmental stage: Exclusively expressed in proliferating cells from the transition G1/S until the end of cytokinesis.
Sequence similarities: Belongs to the TPX2 family.
Research Articles on TPX2
Similar Products
Product Notes
The TPX2 tpx2 (Catalog #AAA515412) is a Recombinant Protein produced from Sf9 insect cells and is intended for research purposes only. The product is available for immediate purchase. The tag for this protein is His tag!!Expression System||Sf9 insect cells using baculovirus!!Source Note||Recombinant full-length human TPX2 protein was expressed by baculovirus in Sf9 insect cells. This product can be used for Aurora An activation in vitro. AAA Biotech's TPX2 can be used in a range of immunoassay formats including, but not limited to, Kinase Assay, Western Blot (WB). Researchers should empirically determine the suitability of the TPX2 tpx2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TPX2, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
