Human Tryptase gamma ELISA Kit | TPSG1 elisa kit

Human Tryptase gamma ELISA Kit

Cat. Num. AAA762138

• Gene Names: TPSG1; TMT; trpA; PRSS31
• Reactivity: Human
• Synonyms: Tryptase gamma; Human Tryptase gamma ELISA Kit; TPSG1/Tryptase gamma -1/gamma I/gamma II/lung tryptase/mast cell protease II/mast cell tryptase/PRSS31skin tryptase/Serine protease 31/Transmembrane tryptase/trpA/tryptase gamma/tryptase gamma 1/tryptase gamma I/tryptase gamma II; TPSG1 elisa kit

• Reactivity: Human
• Sequence Length: 321

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 31.2-2000pg/ml
• Sensitivity: 18.75pg/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for TPSG1 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human TPSG1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human TPSG1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human TPSG1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human TPSG1 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 296453005
• NCBI GeneID: 25823
• Molecular Weight: 33,815 Da
• NCBI Official Full Name: Tryptase gamma
• NCBI Official Synonym Full Names: tryptase gamma 1
• NCBI Official Symbol: TPSG1
• NCBI Official Synonym Symbols: TMT; trpA; PRSS31
• NCBI Protein Information: tryptase gamma
• UniProt Protein Name: Tryptase gamma
• Protein Family: Tryptase
• UniProt Gene Name: TPSG1
• UniProt Synonym Gene Names: PRSS31; TMT
• UniProt Entry Name: TRYG1_HUMAN

NCBI Description

Tryptases comprise a family of trypsin-like serine proteases, the peptidase family S1. Tryptases are enzymatically active only as heparin-stabilized tetramers, and they are resistant to all known endogenous proteinase inhibitors. Several tryptase genes are clustered on chromosome 16p13.3. There is uncertainty regarding the number of genes in this cluster. Currently four functional genes - alpha I, beta I, beta II and gamma I - have been identified. And beta I has an allelic variant named alpha II, beta II has an allelic variant beta III, also gamma I has an allelic variant gamma II. Beta tryptases appear to be the main isoenzymes expressed in mast cells; whereas in basophils, alpha-tryptases predominant. This gene differs from other members of the tryptase gene family in that it has C-terminal hydrophobic domain, which may serve as a membrane anchor. Tryptases have been implicated as mediators in the pathogenesis of asthma and other allergic and inflammatory disorders. [provided by RefSeq, Jul 2008]

Uniprot Description

TPSG1: Tryptases comprise a family of trypsin-like serine proteases, the peptidase family S1. Tryptases are enzymatically active only as heparin-stabilized tetramers, and they are resistant to all known endogenous proteinase inhibitors. Several tryptase genes are clustered on chromosome 16p13.3. There is uncertainty regarding the number of genes in this cluster. Currently four functional genes - alpha I, beta I, beta II and gamma I - have been identified. And beta I has an allelic variant named alpha II, beta II has an allelic variant beta III, also gamma I has an allelic variant gamma II. Beta tryptases appear to be the main isoenzymes expressed in mast cells; whereas in basophils, alpha-tryptases predominant. This gene differs from other members of the tryptase gene family in that it has C-terminal hydrophobic domain, which may serve as a membrane anchor. Tryptases have been implicated as mediators in the pathogenesis of asthma and other allergic and inflammatory disorders. [provided by RefSeq, Jul 2008]

Protein type: EC 3.4.21.-; Membrane protein, integral; Protease

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: integral to plasma membrane

Molecular Function: serine-type endopeptidase activity; serine-type peptidase activity

Biological Process: proteolysis