E3 ubiquitin-protein ligase Topors (Topors) Recombinant Protein | Topors recombinant protein

Recombinant Drosophila melanogaster E3 ubiquitin-protein ligase Topors (Topors), partial

• Gene Names: Topors; CG15104; DmelCG15104; dtopors; dTopors; nbl; TOPORS
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: E3 ubiquitin-protein ligase Topors (Topors); Recombinant Drosophila melanogaster E3 ubiquitin-protein ligase Topors (Topors); partial; Topors recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Length: 1,038

• Species: Drosophila melanogaster (Fruit fly)
• Preparation and Storage: Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.

NCBI and Uniprot Product Information

• NCBI GI #: 442624184
• NCBI GeneID: 37188
• NCBI Accession #: NP_001261083.1
• NCBI GenBank Nucleotide #: NM_001274154.1
• UniProt Accession #: Q9V8P9
• Molecular Weight: 113,313 Da
• NCBI Official Full Name: topoisomerase I-interacting protein, isoform B
• NCBI Official Synonym Full Names: Topoisomerase I-interacting protein
• NCBI Official Symbol: Topors
• NCBI Official Synonym Symbols: CG15104; DmelCG15104; dtopors; dTopors; nbl; TOPORS
• NCBI Protein Information: CG15104 gene product from transcript CG15104-RB
• UniProt Protein Name: E3 ubiquitin-protein ligase Topors
• Protein Family: E3 ubiquitin-protein ligase
• UniProt Gene Name: Topors

Uniprot Description

Functions as a ubiquitin-protein E3 ligase. Negatively regulates the transcriptional repressor h/hairy by promoting its ubiquitination and subsequent degradation. Also directs the nuclear organization of the gypsy chromatin insulator. Chromatin insulators are regulatory elements which establish independent domains of transcriptional activity within eukaryotic genomes. Insulators have two defining properties; they can block the communication between an enhancer and a promoter when placed between them, and can also buffer transgenes from position effect variegation (PEV). Insulators are proposed to structure the chromatin fiber into independent domains of differing transcriptional potential by promoting the formation of distinct chromatin loops. This chromatin looping may require the formation of insulator bodies, where homotypic interactions between individual subunits of the insulator complex could promote the clustering of widely spaced insulators at the nuclear periphery. Within the gypsy insulator complex, this protein may promote formation of nuclear insulator bodies by recruiting individual insulator complexes to the nuclear lamina.