Human Tenascin X ELISA Kit | TNX elisa kit

Human Tenascin X ELISA Kit

Cat. Num. AAA44590

• Gene Names: TNXB; XB; TNX; XBS; EDS3; HXBL; TENX; TN-X; TNXB1; TNXB2; TNXBS
• Reactivity: Human
• Synonyms: Tenascin X; Human Tenascin X ELISA Kit; TNX elisa kit

• Reactivity: Human
• Specificity: This kit recognizes recombinant and natural Human TNX, no significant cross-reactivity or interference was observed.

• Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids
• Detection Range: 3.12 ng/ml - 100 ng/ml.
• Sensitivity: 1.0 ng/ml.
• Intended Uses: This ELISA kit is intended for laboratory in vitro research use only, not for drug, household or other use! The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm by a spectrophotometer. In order to measure the concentration of Human TNX in the sample, this ELISA kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus Human TNX concentration. The concentration of Human TNX in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Intra-assay Precision: Intra-assay CV (%) are less than 15%.
• Inter-assay Precision: Inter-assay CV (%) are less than 15%.
• Preparation and Storage: Store all reagents at 2-8 degree C

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TNX elisa kit

Introduction: ELISA is a simple and highly sensitive method of analysis that allows for simultaneous and rapid quantification of a large number of samples. The assay is based on the specific recognition of the target compound (analyte/antigen) by antibodies which bind to the compound. The antigen-antibody complex is detected and measured with the aid of an enzyme-labeled antibody or antigen. Upon addition of a non-colored reagent, the enzyme produces a color reaction where the color intensity is directly or inversely proportional to the concentration of the analyte in the sample.

NCBI and Uniprot Product Information

• NCBI GI #: 188528648
• NCBI GeneID: 7148
• NCBI Accession #: NP_061978.6
• NCBI GenBank Nucleotide #: NM_019105.6
• UniProt Accession #: P22105; P78530; P78531; Q08424; Q08AM0; Q08AM1; Q59GU7; Q5SQD3; Q5ST74; Q7L8Q4; Q8N4R1; Q9NPK9
• Molecular Weight: 464,325 Da
• NCBI Official Full Name: tenascin-X isoform 1
• NCBI Official Synonym Full Names: tenascin XB
• NCBI Official Symbol: TNXB
• NCBI Official Synonym Symbols: XB; TNX; XBS; EDS3; HXBL; TENX; TN-X; TNXB1; TNXB2; TNXBS
• NCBI Protein Information: tenascin-X; tenascin XB1; tenascin XB2; hexabrachion-like protein; growth-inhibiting protein 45
• UniProt Protein Name: Tenascin-X
• UniProt Gene Name: TNXB
• UniProt Synonym Gene Names: HXBL; TNX; TNXB1; TNXB2; XB; TN-X
• UniProt Entry Name: TENX_HUMAN

NCBI Description

This gene encodes a member of the tenascin family of extracellular matrix glycoproteins. The tenascins have anti-adhesive effects, as opposed to fibronectin which is adhesive. This protein is thought to function in matrix maturation during wound healing, and its deficiency has been associated with the connective tissue disorder Ehlers-Danlos syndrome. This gene localizes to the major histocompatibility complex (MHC) class III region on chromosome 6. It is one of four genes in this cluster which have been duplicated. The duplicated copy of this gene is incomplete and is a pseudogene which is transcribed but does not encode a protein. The structure of this gene is unusual in that it overlaps the CREBL1 and CYP21A2 genes at its 5' and 3' ends, respectively. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

TNXB: Appears to mediate interactions between cells and the extracellular matrix. Substrate-adhesion molecule that appears to inhibit cell migration. Accelerates collagen fibril formation. May play a role in supporting the growth of epithelial tumors. Defects in TNXB are the cause of tenascin-X deficiency (TNXD). TNXD leads to an Ehlers-Danlos-like syndrome characterized by hyperextensible skin, hypermobile joints, and tissue fragility. Tenascin-X-deficient patients, however, lack atrophic scars, a major diagnostic criteria for classic Ehlers- Danlos. Delayed wound healing, which is also common in classic EDS, is only present in a subset of patients. Belongs to the tenascin family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Motility/polarity/chemotaxis; Secreted, signal peptide; Extracellular matrix

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: extracellular matrix; proteinaceous extracellular matrix; extracellular space; fibrillar collagen; intracellular

Molecular Function: heparin binding; integrin binding; collagen binding

Biological Process: elastic fiber assembly; cell-cell adhesion; triacylglycerol metabolic process; collagen fibril organization; collagen metabolic process; cell-matrix adhesion; fibril organization and biogenesis; regulation of JNK activity; actin cytoskeleton organization and biogenesis; cell adhesion; fatty acid metabolic process

Disease: Ehlers-danlos-like Syndrome Due To Tenascin-x Deficiency; Vesicoureteral Reflux 8