Human Transportin 2 ELISA Kit | TNPO2 elisa kit

Human Transportin 2 ELISA Kit

Cat. Num. AAA761437

• Gene Names: TNPO2; IPO3; TRN2; KPNB2B
• Reactivity: Human
• Synonyms: Transportin 2; Human Transportin 2 ELISA Kit; TNPO2; TNPO2 elisa kit

• Reactivity: Human
• Sequence Length: 887

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.313-20ng/ml
• Sensitivity: 0.188ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for TNPO2 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Human TNPO2 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human TNPO2 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human TNPO2 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human TNPO2 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 47938907
• NCBI GeneID: 30000
• Molecular Weight: 100,408 Da
• NCBI Official Full Name: Transportin 2
• NCBI Official Synonym Full Names: transportin 2
• NCBI Official Symbol: TNPO2
• NCBI Official Synonym Symbols: IPO3; TRN2; KPNB2B
• NCBI Protein Information: transportin-2
• UniProt Protein Name: Transportin-2
• Protein Family: Transportin
• UniProt Gene Name: TNPO2
• UniProt Entry Name: TNPO2_HUMAN

Uniprot Description

TNPO2: Probably functions in nuclear protein import as nuclear transport receptor. Serves as receptor for nuclear localization signals (NLS) in cargo substrates. Is thought to mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Belongs to the importin beta family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Nuclear import; Karyopherin

Chromosomal Location of Human Ortholog: 19p13.2

Cellular Component: cytoplasm; nuclear membrane; nucleus

Molecular Function: nuclear localization sequence binding; protein binding; protein transporter activity; Ran GTPase binding

Biological Process: negative regulation of muscle cell differentiation; NLS-bearing substrate import into nucleus; protein import into nucleus, docking; protein import into nucleus, translocation; ribosomal protein import into nucleus