Human Troponin T, slow skeletal muscle ELISA Kit | TNNT1 elisa kit

Human Troponin T, slow skeletal muscle ELISA Kit

Cat. Num. AAA764955

• Gene Names: Tnnt1; sTnT; tnTs; Fang2
• Reactivity: Human
• Synonyms: Troponin T; slow skeletal muscle; Human Troponin T; slow skeletal muscle ELISA Kit; TNNT1 (Troponin T Type 1; Slow Skeletal)/TNT/TnTs/sTnT/Slow skeletal muscle troponin T/Troponin T; TNNT1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of TNNT1 . No significant cross-reactivity or interference between TNNT1 and analogues was observed.
• Sequence Length: 250

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Sandwich
• Detection Range: 15.625-1000pg/ml
• Sensitivity: <9.375pg/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level TNNT1 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level TNNT1 were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TNNT1 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- TNNT1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- TNNT1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the TNNT1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of TNNT1 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 471270250
• NCBI GeneID: 171409
• NCBI Accession #: NP_001264191.1
• NCBI GenBank Nucleotide #: NM_001277262.2
• Molecular Weight: 30,010 Da
• NCBI Official Full Name: troponin T, slow skeletal muscle isoform 4
• NCBI Official Synonym Full Names: troponin T type 1 (skeletal, slow)
• NCBI Official Symbol: Tnnt1
• NCBI Official Synonym Symbols: sTnT; tnTs; Fang2
• NCBI Protein Information: troponin T, slow skeletal muscle
• UniProt Protein Name: Troponin T, slow skeletal muscle
• Protein Family: Troponin
• UniProt Gene Name: Tnnt1
• UniProt Synonym Gene Names: Fang2; TnTs; sTnT
• UniProt Entry Name: TNNT1_RAT

NCBI Description

human homolog plays a role in the calcium sensitivity of contraction in skeletal muscle [RGD, Feb 2006]

Uniprot Description

TNNT1: Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. Defects in TNNT1 are the cause of nemaline myopathy type 5 (NEM5); also known as Amish nemaline myopathy (ANM). A form of nemaline myopathy. Nemaline myopathies are muscular disorders characterized by muscle weakness of varying severity and onset, and abnormal thread-or rod-like structures in muscle fibers on histologic examination. Nemaline myopathy type 5 is a severe and progressive form common among Old Order Amish. Affected infants display tremors with hypotonia and mild contractures of the shoulders and hips. Proximal contractures progressively weaken and a pectus carinatum deformity develops before children die of respiratory insufficiency, usually in the second year. Belongs to the troponin T family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Contractile; Motility/polarity/chemotaxis

Cellular Component: troponin complex

Molecular Function: tropomyosin binding; troponin T binding

Biological Process: negative regulation of muscle contraction; regulation of muscle contraction; skeletal muscle contraction; slow-twitch skeletal muscle fiber contraction; transition between fast and slow fiber