Troponin C, Cardiac, Rabbit Recombinant Protein | TNNC1 recombinant protein

Troponin C, Cardiac, Recombinant, Rabbit (cTnC)

Cat. Num. AAA638261

• Gene Names: TNNC1; TNC; TN-C; TNNC; CMD1Z; CMH13
• Purity: Highly Purified; 95% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified.
• Synonyms: Troponin C; Cardiac; Rabbit; Recombinant; Rabbit (cTnC); TNNC1 recombinant protein

• Host: E Coli
• Purity/Purification: Highly Purified; 95% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified.
• Form/Format: Supplied as a lyophilized powder in 150mM sodium chloride, pH 7.0, 10mM sodium phosphate, 0.5mM EDTA, 0.02% sodium azide.

• Dimers and Aggregates: 1% as determined by silver-stained SDS-PAGE gel analysis.
• Endotoxin Level: 0.1ng/ug (IEU/ug) of Rabbit TNC.
• Protein Content: Protein quantitation was carried out by:; 1-10 mg/ml Bradford assay vs. BSA
• Reconstitution: Reconstitute the lyophilized TNC in sterile 18MÙ-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
• Preparation and Storage: Lyophilized powder may be stored at 4 degree C for short-term only. Reconstitute to nominal volume by adding sterile dH2O and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for TNNC1 recombinant protein

Cardiac troponin C belongs to the EF-hand superfamily of calcium-binding proteins and plays an essential role in the regulation of muscle contraction and relaxation.

Recombinant Rabbit TNC is a single, non-glycosylated, polypeptide chain having a molecular mass of 18,020D.

Product Categories/Family for TNNC1 recombinant protein

Molecular Biology; MB-Cardiac Markers

NCBI and Uniprot Product Information

• NCBI GI #: 4507615
• NCBI GeneID: 7134
• NCBI Accession #: NP_003271.1
• NCBI GenBank Nucleotide #: NM_003280.2
• UniProt Accession #: P63316; O14800; P02590; P04463
• Molecular Weight: 18,403 Da
• NCBI Official Full Name: troponin C, slow skeletal and cardiac muscles
• NCBI Official Synonym Full Names: troponin C type 1 (slow)
• NCBI Official Symbol: TNNC1
• NCBI Official Synonym Symbols: TNC; TN-C; TNNC; CMD1Z; CMH13
• NCBI Protein Information: troponin C, slow skeletal and cardiac muscles; troponin C1, slow; cardiac troponin C; slow twitch skeletal/cardiac muscle troponin C
• UniProt Protein Name: Troponin C, slow skeletal and cardiac muscles
• Protein Family: Troponin
• UniProt Gene Name: TNNC1
• UniProt Synonym Gene Names: TNNC; TN-C
• UniProt Entry Name: TNNC1_HUMAN

NCBI Description

Troponin is a central regulatory protein of striated muscle contraction, and together with tropomyosin, is located on the actin filament. Troponin consists of 3 subunits: TnI, which is the inhibitor of actomyosin ATPase; TnT, which contains the binding site for tropomyosin; and TnC, the protein encoded by this gene. The binding of calcium to TnC abolishes the inhibitory action of TnI, thus allowing the interaction of actin with myosin, the hydrolysis of ATP, and the generation of tension. Mutations in this gene are associated with cardiomyopathy dilated type 1Z. [provided by RefSeq, Oct 2008]

Uniprot Description

TNNC1: Troponin is the central regulatory protein of striated muscle contraction. Tn consists of three components: Tn-I which is the inhibitor of actomyosin ATPase, Tn-T which contains the binding site for tropomyosin and Tn-C. The binding of calcium to Tn-C abolishes the inhibitory action of Tn on actin filaments. Defects in TNNC1 are the cause of cardiomyopathy dilated type 1Z (CMD1Z). Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death. Defects in TNNC1 are the cause of familial hypertrophic cardiomyopathy type 13 (CMH13). A hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death. Belongs to the troponin C family.

Protein type: Calcium-binding; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 3p21.1

Cellular Component: nucleoplasm; troponin complex; mitochondrion; cytosol; actin cytoskeleton

Molecular Function: troponin T binding; actin filament binding; protein binding; protein homodimerization activity; troponin I binding; calcium ion binding; calcium-dependent protein binding

Biological Process: response to metal ion; regulation of ATPase activity; regulation of muscle contraction; diaphragm contraction; ventricular cardiac muscle morphogenesis; regulation of muscle filament sliding speed; muscle filament sliding; cardiac muscle contraction

Disease: Cardiomyopathy, Dilated, 1z; Cardiomyopathy, Familial Hypertrophic, 13