Rat Activated CDC42 kinase 1 ELISA Kit | TNK2 elisa kit

Rat Activated CDC42 kinase 1 (TNK2) ELISA Kit

Cat. Num. AAA280691

• Reactivity: Rat
• Synonyms: Activated CDC42 kinase 1; Rat Activated CDC42 kinase 1 (TNK2) ELISA Kit; TNK2 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Rat TNK2. No significant cross-reactivity or interference between Rat TNK2 and analogues was observed.
• Sequence Length: 1038

• Samples: Serum, Plasma, Other biological fluids
• Assay Type: Sandwich
• Sample Volume: 1-200 uL
• Precision: Intra-assay Precision (Precision within an assay); Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.; Inter-assay Precision (Precision between assays); Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.; CV (%) = SD/meanX100; Intra-Assay: CV; Inter-Assay: CV
• Detection Wavelength: 450 nm
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for TNK2 elisa kit

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate TNK2 in samples. An antibody specific for TNK2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTNK2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNK2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNK2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 56605750
• NCBI GeneID: 303882
• NCBI Accession #: NP_001008337.1
• NCBI GenBank Nucleotide #: NM_001008336.1
• UniProt Accession #: Q5U2X5
• Molecular Weight: 115,068 Da
• NCBI Official Full Name: activated CDC42 kinase 1
• NCBI Official Synonym Full Names: tyrosine kinase, non-receptor, 2
• NCBI Official Symbol: Tnk2
• NCBI Protein Information: activated CDC42 kinase 1
• UniProt Protein Name: Activated CDC42 kinase 1
• Protein Family: Activated CDC42 kinase
• UniProt Gene Name: Tnk2
• UniProt Synonym Gene Names: ACK-1

Uniprot Description

Ack: a tyrosine kinase that binds Cdc42 in its GTP-bound form and inhibits both the intrinsic and GTPase-activating protein (GAP)-stimulated GTPase activity of Cdc42. This binding is mediated by a unique sequence of 47 amino acids C-terminal to an SH3 domain. May be involved in a regulatory mechanism that sustains the GTP-bound active form of Cdc42 and which is directly linked to a tyrosine phosphorylation signal transduction pathway. Amplification in primary tumors correlates with metastatic potential . Two differentially spliced isoforms have been described.

Protein type: Ack family; EC 2.7.10.2; EC 2.7.11.1; Kinase, protein; Protein kinase, TK; Protein kinase, tyrosine (non-receptor); TK group

Chromosomal Location of Human Ortholog: 11q22

Cellular Component: adherens junction; axon; cell soma; clathrin-coated pit; clathrin-coated vesicle; cytoplasm; cytoplasmic vesicle membrane; dendrite; endosome; extrinsic component of cytoplasmic side of plasma membrane; growth cone; membrane; nucleus; plasma membrane

Molecular Function: ATP binding; epidermal growth factor receptor binding; identical protein binding; metal ion binding; non-membrane spanning protein tyrosine kinase activity; protein serine/threonine kinase activity; protein serine/threonine/tyrosine kinase activity; protein tyrosine kinase activity; receptor binding; ubiquitin protein ligase binding; WW domain binding

Biological Process: cell differentiation; cell migration; endocytosis; innate immune response; peptidyl-tyrosine phosphorylation; phosphorylation; positive regulation of peptidyl-tyrosine phosphorylation; regulation of cell proliferation; signal transduction; transmembrane receptor protein tyrosine kinase signaling pathway