CD30L Recombinant Protein | TNFSF8 recombinant protein

CD30L Protein, Human, Recombinant (hFc Tag)

Cat. Num. AAA8118584

• Gene Names: TNFSF8; CD153; CD30L; CD30LG
• Purity: >95 % as determined by SDS-PAGE
• Synonyms: CD30L; CD30L Protein; Human; Recombinant (hFc Tag); Human CD153/CD30L/TNFSF8 Protein (Fc Tag); tumor necrosis factor (ligand) superfamily; member 8; CD153 Protein; CD30LG Protein; TNFSF8 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >95 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4 Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Sequence Positions: 429aa
• Sequence: Gln63-Asp234

• Species: Human
• Tag: hFc
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Arg23
• Protein Construction: |A DNA sequence encoding the extracellular domain (Gln 63-Asp 234) of human CD30 Ligand (NP_001235.1) was fused with the Fc region of human IgG1 at the N-terminus
• Activity: Measured by its binding ability in a functional ELISA. Immobilized human CD30L-hFc at 2ug/ml (100ul/well) can bind human CD30/TNFRSF8-Fch. The EC50 of human CD30/TNFRSF8-Fch is 15-60ng/mL.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; Shipping: They are shipped out with blue ice pack

Testing Data

Testing Data

(Measured by its binding ability in a functional ELISA. Immobilized human CD30L-hFc (Cat: 10040-H01H) at 2 ug/ml (100 ul/well) can bind human CD30/TNFRSF8-Fch (Cat: 10777-H03H), the EC50 of human CD30/TNFRSF8-Fch is 15-60 ng/mL.)

Related Product Information for TNFSF8 recombinant protein

CD30 ligand (CD30L), also known as CD153 and TNFSF8, is a membrane-associated glycoprotein belonging to the TNF superfamily and TNFR superfamily, and is a specific ligand for CD30/TNFRSF8 originally described as a cell surface antigen and a marker for Hodgkin lymphoma and related hematologic malignancies. CD30L is a type-II membrane glycoprotein expressed on activated T cells, stimulated monocyte-macrophages, granulocytes, eosinophils, and some Burkitt-like lymphoma cell lines. CD30L is capable of transducing signals through CD30 on different CD30+ lymphoma cell lines, and mediates pleiotropic biologic effects including cell proliferation, activation, differentiation, as well as cell death by apoptosis. CD30-CD30 ligand interaction has been suggested to have a pathophysiologic role in malignant lymphomas, particularly Hodgkin disease, large cell anaplastic lymphomas and Burkitt lymphomas, and is also involved in activation and functioning of the T cell-dependent immune response. Thus, CD153 and its receptor CD30 are regarded as therapeutic targets in hematologic malignancies, autoimmune and inflammatory diseases.

References

Hargreaves PG, et al. (2002) Soluble CD30 binds to CD153 with high affinity and blocks transmembrane signaling by CD30. Eur J Immunol. 32(1): 163-73.Blazar BR, et al. (2004) CD30/CD30 ligand (CD153) interaction regulates CD4+ T cell-mediated graft-versus-host disease. J Immunol. 173(5): 2933-41.Oflazoglu E, et al. (2009) Targeting CD30/CD30L in oncology and autoimmune and inflammatory diseases. Adv Exp Med Biol. 647: 174-85.

NCBI and Uniprot Product Information

• NCBI GI #: 4507607
• NCBI GeneID: 944
• NCBI Accession #: NP_001235.1
• NCBI GenBank Nucleotide #: NM_001244.3
• UniProt Accession #: P32971; O43404
• Molecular Weight: 26,017 Da
• NCBI Official Full Name: tumor necrosis factor ligand superfamily member 8 isoform 1
• NCBI Official Synonym Full Names: tumor necrosis factor (ligand) superfamily, member 8
• NCBI Official Symbol: TNFSF8
• NCBI Official Synonym Symbols: CD153; CD30L; CD30LG
• NCBI Protein Information: tumor necrosis factor ligand superfamily member 8; CD30-L; CD30 ligand; CD153 antigen; CD30 antigen ligand
• UniProt Protein Name: Tumor necrosis factor ligand superfamily member 8
• Protein Family: Tumor necrosis factor ligand superfamily
• UniProt Gene Name: TNFSF8
• UniProt Synonym Gene Names: CD30L; CD30LG; CD30-L
• UniProt Entry Name: TNFL8_HUMAN

NCBI Description

The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for TNFRSF8/CD30, which is a cell surface antigen and a marker for Hodgkin lymphoma and related hematologic malignancies. The engagement of this cytokine expressed on B cell surface plays an inhibitory role in modulating Ig class switch. This cytokine was shown to enhance cell proliferation of some lymphoma cell lines, while to induce cell death and reduce cell proliferation of other lymphoma cell lines. The pleiotropic biologic activities of this cytokine on different CD30+ lymphoma cell lines may play a pathophysiologic role in Hodgkin's and some non-Hodgkin's lymphomas. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011]

Uniprot Description

TNFSF8: Cytokine that binds to TNFRSF8/CD30. Induces proliferation of T-cells. Belongs to the tumor necrosis factor family.

Protein type: Apoptosis; Cytokine; Membrane protein, integral

Chromosomal Location of Human Ortholog: 9q33

Cellular Component: extracellular space; integral to plasma membrane

Molecular Function: cytokine activity; tumor necrosis factor receptor binding; receptor binding

Biological Process: cell proliferation; CD8-positive, alpha-beta T cell differentiation; defense response to Gram-positive bacterium; cell-cell signaling; apoptosis; immune response; positive regulation of transcription from RNA polymerase II promoter; signal transduction