TNFRSF4 / OX40 / CD134 recombinant protein

Recombinant Human TNFRSF4 / OX40 / CD134 Protein (His tag)

Cat. Num. AAA2546230

• Gene Names: TNFRSF4; OX40; ACT35; CD134; IMD16; TXGP1L
• Purity: > 90 % as determined by SDS-PAGE
• Synonyms: TNFRSF4 / OX40 / CD134; Recombinant Human TNFRSF4 / OX40 / CD134 Protein (His tag); TNFRSF4; RP5-902P8.3; ACT35; CD134; OX40; TXGP1L; TNFRSF4 / OX40 / CD134 recombinant protein

• Host: Human Cells
• Purity/Purification: > 90 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 277

• Application Notes: The recombinant human TNFRSF4 consists of 199 amino acids after removal of the signal peptide and has a predicted molecular mass of 21.7 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh TNFRSF4 is approximately 40-45 kDa due to glycosylation.
• Predicted N Terminal: Leu 29
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for TNFRSF4 / OX40 / CD134 recombinant protein

Background: OX40 (CD134) and its binding partner, OX40L (CD252), are members of the tumor necrosis factor receptor/tumor necrosis factor superfamily, is known to break an existing state of tolerance in malignancies, leading to a reactivation of antitumor immunity. The interaction between OX40 and OX40L plays an important role in antigen-specific T-cell expansion and survival. OX40 and OX40L also regulate cytokine production from T cells, antigen-presenting cells, natural killer cells, and natural killer T cells, and modulate cytokine receptor signaling. In line with these important modulatory functions, OX40-OX40L interactions have been found to play a central role in the development of multiple inflammatory and autoimmune diseases, making them attractive candidates for intervention in the clinic. Conversely, stimulating OX40 has shown it to be a candidate for therapeutic immunization strategies for cancer and infectious disease.

Description: A DNA sequence encoding the extracellular domain (Met 1-Ala 216) of human TNFRSF4 (NP_003318.1) was expressed, fused with a polyhistidine tag at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 4507579
• NCBI GeneID: 7293
• NCBI Accession #: NP_003318.1
• NCBI GenBank Nucleotide #: NM_003327.3
• UniProt Accession #: P43489; Q13663; Q2M312; Q5T7M0
• Molecular Weight: 29,341 Da
• NCBI Official Full Name: tumor necrosis factor receptor superfamily member 4
• NCBI Official Synonym Full Names: TNF receptor superfamily member 4
• NCBI Official Symbol: TNFRSF4
• NCBI Official Synonym Symbols: OX40; ACT35; CD134; IMD16; TXGP1L
• NCBI Protein Information: tumor necrosis factor receptor superfamily member 4
• UniProt Protein Name: Tumor necrosis factor receptor superfamily member 4
• UniProt Gene Name: TNFRSF4
• UniProt Synonym Gene Names: TXGP1L

NCBI Description

The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor has been shown to activate NF-kappaB through its interaction with adaptor proteins TRAF2 and TRAF5. Knockout studies in mice suggested that this receptor promotes the expression of apoptosis inhibitors BCL2 and BCL2lL1/BCL2-XL, and thus suppresses apoptosis. The knockout studies also suggested the roles of this receptor in CD4+ T cell response, as well as in T cell-dependent B cell proliferation and differentiation. [provided by RefSeq, Jul 2008]

Uniprot Description

TNFRSF4: Receptor for TNFSF4/OX40L/GP34.

Protein type: Apoptosis; Membrane protein, integral; Receptor, misc.

Chromosomal Location of Human Ortholog: 1p36.33

Cellular Component: cell surface; integral to plasma membrane; plasma membrane

Molecular Function: tumor necrosis factor receptor activity

Biological Process: immune response; inflammatory response; multicellular organismal development; negative regulation of transcription factor activity; negative regulation of transcription, DNA-dependent; positive regulation of B cell proliferation; positive regulation of immunoglobulin secretion; positive regulation of MAPKKK cascade; regulation of apoptosis; regulation of cell proliferation; response to lipopolysaccharide; T cell proliferation; tumor necrosis factor-mediated signaling pathway

Disease: Immunodeficiency 16