TNF recombinant protein
Recombinant Rabbit TNF Protein
Gene Names
TNF; TNFA; TNFSF2; TNLG1F
Reactivity
Rabbit
Applications
ELISA, Western Blot, SDS-Page
Purity
Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE.
Synonyms
TNF; Recombinant Rabbit TNF Protein; TNFA; TNFSF2; Tumor necrosis factor; Cachectin; TNF-alpha; Tumor necrosis factor ligand superfamily member 2; TNF-a; TNF recombinant protein
Host
E Coli
Reactivity
Rabbit
Purity/Purification
Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE.
Form/Format
Lyophilized from a 0.2 muM filtered solution of 20mM PB, 300mM NaCl, pH 7.4.
Sequence
MLRSASRALS DKPLAHVVAN PQVEGQLQWL SQRANALLAN GMKLTDNQLV VPADGLYLIY SQVLFSGQGC RSYVLLTHTV SRFAVSYPNK VNLLSAIKSP CHRETPEEAE PMAWYEPIYL GGVFQLEKGD RLSTEVNQPE YLDLAESGQV YFGIIAL
Sequence Length
234
Applicable Applications for TNF recombinant protein
ELISA (EIA),Western Blot (WB), SDS-PAGE, Mass Spectrometry (MS)
Endotoxin
Less than 0.1 ng/mug (1 IEU/mug) as determined by LAL test.
Directions for Use
Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 mug/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Preparation and Storage
Lyophilized protein should be stored at -20 degree C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8 degree C for 2-7 days. Aliquots of reconstituted samples are stable at -20 degree C for 3 months.
Related Product Information for TNF recombinant protein
Recombinant Rabbit TNF Protein is produced by E Coli using rDNA technology. The protein consists of three identical polypeptide chains of 158 amino acids combined to form a compact, bell-shaped homotrimer. The individual subunits have a relative molecular mass each of 17.4 Daltons.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
25,817 Da
NCBI Official Full Name
tumor necrosis factor
NCBI Official Symbol
TNF
NCBI Official Synonym Symbols
TNFA; TNFSF2; TNLG1F
NCBI Protein Information
tumor necrosis factor
UniProt Protein Name
Tumor necrosis factor
Protein Family
UniProt Gene Name
TNF
UniProt Synonym Gene Names
TNFA; TNFSF2; TNF-a; NTF; ICD1; ICD2
UniProt Entry Name
TNFA_RABIT
Uniprot Description
Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells.
Research Articles on TNF
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Product Notes
The TNF tnf (Catalog #AAA8249892) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The Recombinant Rabbit TNF Protein reacts with Rabbit and may cross-react with other species as described in the data sheet. AAA Biotech's TNF can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA),Western Blot (WB), SDS-PAGE, Mass Spectrometry (MS). Researchers should empirically determine the suitability of the TNF tnf for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MLRSASRALS DKPLAHVVAN PQVEGQLQWL SQRANALLAN GMKLTDNQLV VPADGLYLIY SQVLFSGQGC RSYVLLTHTV SRFAVSYPNK VNLLSAIKSP CHRETPEEAE PMAWYEPIYL GGVFQLEKGD RLSTEVNQPE YLDLAESGQV YFGIIAL. It is sometimes possible for the material contained within the vial of "TNF, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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