Typical Testing Data/Standard Curve (for reference only)
Canine Thymosin Beta 10 ELISA Kit | TMSbeta10 elisa kit
Canine Thymosin Beta 10 ELISA Kit
Gene Names
TMSB10; TB10; MIG12
Reactivity
Canine
Synonyms
Thymosin Beta 10; Canine Thymosin Beta 10 ELISA Kit; TMSbeta10 elisa kit
Reactivity
Canine
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Detection Range
25-500ng/mL
Sensitivity
1.0ng/mL
Intended Uses
This TMSB10 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine TMSb10. This ELISA kit for research use only, not for therapeutic applications!
Preparation and Storage
Store all reagents at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for TMSbeta10 elisa kit
Principle of the Assay: TMSB10 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-TMSB10 antibody and an TMSB10-HRP conjugate. The assay sample and buffer are incubated together with TMSB10-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TMSB10 concentration since TMSB10 from samples and TMSB10-HRP conjugate compete for the anti-TMSB10 antibody binding site. Since the number of sites is limited, as more sites are occupied by TMSB10 from the sample, fewer sites are left to bind TMSB10-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TMSB10 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for TMSbeta10 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
5,026 Da
NCBI Official Full Name
thymosin beta-10
NCBI Official Synonym Full Names
thymosin beta 10
NCBI Official Symbol
TMSB10
NCBI Official Synonym Symbols
TB10; MIG12
NCBI Protein Information
thymosin beta-10; migration-inducing gene 12; migration-inducing protein 12
UniProt Protein Name
Thymosin beta-10
UniProt Gene Name
TMSB10
UniProt Synonym Gene Names
PTMB10; THYB10
UniProt Entry Name
TYB10_HUMAN
Uniprot Description
TMSB10: Plays an important role in the organization of the cytoskeleton. Binds to and sequesters actin monomers (G actin) and therefore inhibits actin polymerization. Belongs to the thymosin beta family.
Protein type: Motility/polarity/chemotaxis; Cytoskeletal
Chromosomal Location of Human Ortholog: 2p11.2
Cellular Component: cytoskeleton; cytoplasm
Molecular Function: actin monomer binding
Biological Process: sequestering of actin monomers; actin filament organization
Research Articles on TMSbeta10
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Product Notes
The Canine TMSbeta10 tmsb10 (Catalog #AAA743822) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA743822 ELISA Kit recognizes Canine TMSbeta10. It is sometimes possible for the material contained within the vial of "Thymosin Beta 10, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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